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Method for acquiring hematopoietic stem cells from placenta in low-oxygen environment

A technology of hematopoietic stem cells and oxygen environment, applied in cell dissociation methods, blood/immune system cells, animal cells, etc., can solve the problem of underestimation of the number of stem cells, and achieve the goal of overcoming non-physiological oxygen stress, increasing the number, and maintaining a primitive state. Effect

Active Publication Date: 2018-12-07
希瑞干细胞科技有限公司
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Problems solved by technology

However, so far, almost all research results on hematopoietic stem cells are based on the partial pressure of oxygen under non-physiological conditions, i.e. normoxia, and the number of stem cells obtained under normoxia is often underestimated

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  • Method for acquiring hematopoietic stem cells from placenta in low-oxygen environment
  • Method for acquiring hematopoietic stem cells from placenta in low-oxygen environment
  • Method for acquiring hematopoietic stem cells from placenta in low-oxygen environment

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Embodiment Construction

[0032] The invention discloses a method for obtaining hematopoietic stem cells from placenta in a hypoxic environment. Those skilled in the art can learn from the content of this article and appropriately improve the process parameters to realize it. In particular, it should be pointed out that all similar replacements and modifications are obvious to those skilled in the art, and they are all considered to be included in the present invention. The method and application of the present invention have been described through preferred embodiments, and relevant personnel can obviously make changes or appropriate changes and combinations to the methods described herein without departing from the content, spirit and scope of the present invention to realize and apply the present invention Invent technology.

[0033] Preliminary preparations are as follows:

[0034] 1. Source of placental tissue

[0035] There is no history of hereditary diseases and congenital diseases in the famil...

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Abstract

The invention relates to the technical field of hematopoietic stem cell acquiring methods, and particularly relates to a method for acquiring hematopoietic stem cells from placenta in a low-oxygen environment. According to the method, the process of acquiring hematopoietic stem cells from placenta is completely finished in a simulated organism under the condition of a normal low-oxygen environment; and a digestive juice used in the process of acquiring the hematopoietic stem cell is a mixed juice of collagenase, dispase, DNA enzyme 1 and fetal calf serum. By adopting the method of acquiring the hematopoietic stem cells from placenta, the problem of unphysiological oxygen pressure stress caused by short hyperoxia can be overcome, and the hematopoietic stem cells with large number and original state can be acquired and have excellent proliferating and differentiative capacity in vitro.

Description

technical field [0001] The invention relates to the technical field of methods for obtaining hematopoietic stem cells, in particular to a method for obtaining hematopoietic stem cells from placenta in a hypoxic environment. Background technique [0002] Hematopoietic stem cells (HSCs) are a small group of hematopoietic precursor cells with high self-renewal and multilineage differentiation potential. It has been confirmed that HSC can differentiate into at least 12 series of blood cells and other cells, including red blood cells, granulocytes, monocytes, eosinophils, basophils, mast cells, platelets, total lymphocytes, B lymphocytes, Dendritic cells, natural killer cells, and osteoblasts and fibroblasts in the bone marrow. Hematopoietic stem cell transplantation is an important means to treat malignant blood diseases and some immune diseases. Hematopoietic stem cells are generally derived from bone marrow and umbilical cord blood. [0003] Allogeneic bone marrow hematopoi...

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Application Information

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IPC IPC(8): C12N5/0789
CPCC12N5/0647C12N2509/00C12N2500/02
Inventor 王浩朱丰城龚霄翔季夏芸
Owner 希瑞干细胞科技有限公司
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