Method for predicting hypertension secondary ventricular hypertrophy and detection reagent kit therefor

A technology for hypertension and left ventricle, applied in the field of medical molecular biology, can solve the problems of blood stasis, increased risk of stroke, and promotion of thrombosis, achieve high representativeness, improve feasibility, reliability, and sensitivity and specific effects

Inactive Publication Date: 2008-11-12
FUWAI HOSPITAL OF CARDIOVASCULAR DESEASE CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that left ventricular hypertrophy can easily lead to blood stasis in the heart, easily cause arrhythmias such as atrial fibrillation, promote thrombosis, and increase the risk of ischemic stroke by 2.5 times

Method used

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  • Method for predicting hypertension secondary ventricular hypertrophy and detection reagent kit therefor
  • Method for predicting hypertension secondary ventricular hypertrophy and detection reagent kit therefor
  • Method for predicting hypertension secondary ventricular hypertrophy and detection reagent kit therefor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] This study is a sub-project of investigation and research on the prevalence of left ventricular hypertrophy and related risk factors in hypertensive population in rural China. We detected 5,421 patients with essential hypertension aged 40 to 75 from 7 natural villages in Xinyang, Henan Province by random cluster sampling method, and performed echocardiography on 4,869 of them. From a total of 7 natural villages, we randomly selected 3, and all hypertensive patients who had undergone echocardiographic detection were included in this study, excluding all other cardiovascular diseases affecting left ventricular hypertrophy (hypertrophic cardiomyopathy, valvular heart disease, pulmonary hypertension, and coronary artery disease), resulting in a study population of 2,179 (767 men, 1412 women) patients with essential hypertension. The control group and the case group were enrolled in each center at the same time, aged between 40 and 74 years old, the control group and the cas...

Embodiment 2

[0106] Example 2: A kit for predicting left ventricular hypertrophy secondary to hypertension

[0107] 1. Composition: The composition and content are as follows (50 servings), stored at -20°C:

[0108] 50 μL 10×PCR buffer (TaKaRa),

[0109] 50μL 10mmol / L dNTP mixture (TaKaRa),

[0110] 12 μL (5U / uL) Taq DNA polymerase (TaKaRa),

[0111] Each 20μL (10umol / L) of SEQ ID NO.1 and SEQ ID NO.2 primers (Beijing Aoke Biological Company)

[0112] 5ml deionized double distilled water (ddH 2 O),

[0113] 100 μL 10×Ban II restriction endonuclease reaction buffer (New England Biolabs, USA)

[0114] 20 μL (10U / μL) restriction endonuclease Ban II (New England Biolabs, USA)

[0115] 2. How to use:

[0116] 1. Extract the subject's genomic DNA by conventional methods;

[0117] 2. PCR reaction:

[0118] Table 5: PCR reaction system

[0119]

[0120] Reaction conditions:

[0121] step 1=94.0℃ for 5 minutes

[0122] Repeat for 35 cycles

[0123] step 5=72.0℃ for 7 minutes

[0...

Embodiment 3

[0133] Example 3: Another independent sample validates the endothelial nitric oxide synthase kit for predicting left ventricular hypertrophy secondary to hypertension

[0134] The cases in this study come from the community population in Rizhao, Shandong, and a total of 343 hypertensive patients were selected. The diagnostic criteria for hypertension are based on the seventh report of the United States Joint National Committee on the Prevention, Detection, Evaluation, and Treatment of Hypertension (JNC-7): systolic blood pressure ≥ 140mmHg and / or diastolic blood pressure ≥ 90mmHg, or taking antihypertensive drugs. Patients in each group were excluded from secondary hypertension, coronary heart disease, diabetes mellitus, valvular heart disease, primary pulmonary hypertension heart failure, severe cardiovascular and cerebrovascular diseases, liver and kidney insufficiency, and endocrine system through detailed medical history inquiry, physical examination and laboratory examinat...

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Abstract

The invention discloses a method for forecasting hypertension secondary left ventricular hypertrophy and a detection reagent kit, belonging to the medical molecular biology field. The invention also discloses use of a polymorphic locus of an endothelial nitric oxide synthase gene in forecasting the hypertension secondary left ventricular hypertrophy. The method and the detection reagent kit as well as the use have the advantages that: the function of endothelial nitric oxide synthase gene polymorphism in the hypertension secondary left ventricular hypertrophy is confirmed, a related SNP is utilized to provide a drug target for the treatment of the hypertension secondary left ventricular hypertrophy, and a guideline is provided for personalized diagnosis and prognosis.

Description

technical field [0001] The present invention relates to a method and detection kit for predicting left ventricular hypertrophy secondary to hypertension, more specifically, the role of endothelial nitric oxide synthase gene polymorphism in predicting left ventricular hypertrophy secondary to hypertension in Chinese The use belongs to the field of medical molecular biology. Background technique [0002] Left ventricular hypertrophy (LVH) is the main direct complication of hypertension. According to the survey, among the 4270 hypertensive patients in rural areas of our country, those with heart hypertrophy accounted for 42.8%. Left ventricular hypertrophy is recognized as a precursor to heart failure and an important independent risk factor for heart failure, stroke, coronary heart disease and sudden death. Studies have shown that left ventricular hypertrophy can easily lead to blood stasis in the heart, atrial fibrillation and other arrhythmias, promote thrombosis, and incr...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 惠汝太辛颖宋晓东王晓建刘哲白永怿
Owner FUWAI HOSPITAL OF CARDIOVASCULAR DESEASE CHINESE ACAD OF MEDICAL SCI
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