A method for harvesting hematopoietic stem cells from the placenta in a hypoxic environment

A technology of hematopoietic stem cells and oxygen environment, applied in cell dissociation methods, blood/immune system cells, animal cells, etc., can solve the problem of underestimation of the number of stem cells, and achieve the goal of overcoming non-physiological oxygen stress, a primitive state, and a large number of Effect

Active Publication Date: 2022-01-14
希瑞干细胞科技有限公司
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Problems solved by technology

However, so far, almost all research results on hematopoietic stem cells are based on the partial pressure of oxygen under non-physiological conditions, i.e. normoxia, and the number of stem cells obtained under normoxia is often underestimated

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  • A method for harvesting hematopoietic stem cells from the placenta in a hypoxic environment
  • A method for harvesting hematopoietic stem cells from the placenta in a hypoxic environment
  • A method for harvesting hematopoietic stem cells from the placenta in a hypoxic environment

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Embodiment Construction

[0032] The invention discloses a method for obtaining hematopoietic stem cells from placenta in a hypoxic environment. Those skilled in the art can learn from the content of this article and appropriately improve the process parameters to realize it. In particular, it should be pointed out that all similar replacements and modifications are obvious to those skilled in the art, and they are all considered to be included in the present invention. The method and application of the present invention have been described through preferred embodiments, and relevant personnel can obviously make changes or appropriate changes and combinations to the methods described herein without departing from the content, spirit and scope of the present invention to realize and apply the present invention Invent technology.

[0033] The preliminary preparations are as follows:

[0034] 1. Source of placental tissue

[0035] There is no history of hereditary diseases and congenital diseases in the f...

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Abstract

The invention relates to the technical field of methods for obtaining hematopoietic stem cells, in particular to a method for obtaining hematopoietic stem cells from placenta in a hypoxic environment. The process of obtaining the hematopoietic stem cells in the placenta of the present invention is all completed under the normal hypoxic environment conditions in a simulated organism; serum mixture. The method for obtaining hematopoietic stem cells from the placenta of the present invention overcomes the problem of non-physiological oxygen pressure stress caused by transient hyperoxia, and the obtained hematopoietic stem cells are large in number, primitive in state, and have good proliferation and differentiation capabilities in vitro.

Description

technical field [0001] The invention relates to the technical field of methods for obtaining hematopoietic stem cells, in particular to a method for obtaining hematopoietic stem cells from placenta in a hypoxic environment. Background technique [0002] Hematopoietic stem cells (HSCs) are a small group of hematopoietic precursor cells with high self-renewal and multilineage differentiation potential. It has been confirmed that HSC can differentiate into at least 12 series of blood cells and other cells, including red blood cells, granulocytes, monocytes, eosinophils, basophils, mast cells, platelets, total lymphocytes, B lymphocytes, Dendritic cells, natural killer cells, and osteoblasts and fibroblasts in the bone marrow. Hematopoietic stem cell transplantation is an important means to treat malignant blood diseases and some immune diseases. Hematopoietic stem cells are generally derived from bone marrow and umbilical cord blood. [0003] Allogeneic bone marrow hematopoi...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0789
CPCC12N5/0647C12N2509/00C12N2500/02
Inventor 王浩朱丰城龚霄翔季夏芸
Owner 希瑞干细胞科技有限公司
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