A Chromium-Ion Cutting General-Purpose Partition Ultrafast Amplification Visualization Sensor

A technology of chromium ions and sensors, applied in the field of biosensors
CN108949934BActive Publication Date: 2020-07-28CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CHINA AGRI UNIV
Publication Date
2020-07-28

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Abstract

The invention provides a cleavage type universal-partition ultrafast-amplification visual sensor for chromium ions. The visual sensor comprises (1) a cleavage system, (2) a sPCR amplification system and (3) a detection system containing an ABTS developing solution. According to the invention, primers, a template and probes are ingeniously designed, so ultrafast amplification of the template can berealized in the presence of chromium ions, and an amplification product is allowed to form a G-quadruplex in a proper environment; and color development is performed by utilizing the peroxidase-likeactivity of the G-quadruplex. Thus, the problem of difficulty in realizing visual detection of traditional PCR products is overcome, and rapid and visual detection of chromium ions is realized. Moreover, the sensor provided in the invention has the characteristics of high specificity and high sensitivity to chromium ions, and is more objective and accurate in detection results.
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Description

Technical field

[0001] The invention relates to the technical field of biosensors, in particular to a chromium ion cutting type universal cut-off ultra-fast amplification visualization sensor. Background technique

[0002] Chromium symbol Cr, silver-white metal, belongs to group VIB in the periodic table of elements, chromium has atomic number 24, atomic weight 51.996, body-centered cubic crystal, and common valences are +3, +6 and +2. The average content of chromium in the earth's crust is 0.010% to 0.011%, and the distribution is extremely uneven. my country's chromium resources are not abundant, and trivalent chromium is one of the indispensable trace elements in the human body. Adults need about 500-700 micrograms of chromium per day. When chromium is deficient, the human body will easily suffer from diabetes and coronary atherosclerosis, which will affect the fat metabolism in the body. However, too much chromium can also cause pollution. The toxicity of hexavalent chromi...

Claims

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