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A method for efficiently obtaining aseptic materials of hybrid orchids by utilizing new lateral buds

A technology of hybrid orchids and side buds, applied in the field of plant tissue culture, can solve the problems of unsatisfactory adaptability of imported varieties, high production and operation costs of florists and enterprises, and achieve convenient and quick cutting process, reduce the chance of infection, and increase the chance of pollution Effect

Active Publication Date: 2021-11-12
HANGZHOU ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the hybrid orchid varieties currently circulating in the domestic market are basically imported from abroad, and the number of self-fertile varieties that are commercialized is almost zero. The source of seedlings is controlled by others. unsatisfactory sex

Method used

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  • A method for efficiently obtaining aseptic materials of hybrid orchids by utilizing new lateral buds
  • A method for efficiently obtaining aseptic materials of hybrid orchids by utilizing new lateral buds

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Experimental program
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Embodiment

[0021] 1. Pretreatment. One week before the material collection, the hybrid orchid plants were treated with 800 times carbendazim solution for root irrigation twice with an interval of 3 days, and the explants were guaranteed to be dry when the materials were collected.

[0022] 2. Sampling. Select healthy new shoots from the strong mother plant, remove impurities and dirt, and bring them back to the laboratory.

[0023] 3. Clean the surface. Peel off the old leaves, scrape off the dirt at the base of the root, brush the surface of the explant with a soft brush dipped in detergent, immerse in 75% alcohol for a few seconds after rinsing, immerse in thick soapy water, shake and clean for 1 hour, and rinse with running water for 1 hour Hour.

[0024] 4. Aseptic disinfection. Blot the moisture on the surface of the explants with absorbent paper, transfer them to an ultra-clean workbench for sterilization, and use alcohol, mercury chloride, sodium hypochlorite and other disinfe...

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Abstract

The invention discloses a method for efficiently obtaining hybrid orchid aseptic materials by utilizing new lateral buds, and the method relates to the field of plant tissue culture. The present invention creates a method for improving the survival rate of small lateral buds in new buds of hybrid orchids. Through the combined use of sterilizers, the pollution rate in the aseptic disinfection process is reduced, and the acquisition rate of small lateral buds is improved, which is a specific method for hybrid orchid breeding. Single plant seedling multiplication lays the foundation. The invention uses the small lateral buds produced by the new buds of the hybrid orchids as explants to obtain aseptic materials, which can not only reduce the pollution rate, but also increase the survival rate, and is of great significance for the propagation of hybrid orchid specific single plant seedlings.

Description

technical field [0001] The invention discloses a method for efficiently obtaining hybrid orchid aseptic materials by utilizing new lateral buds, and the method relates to the field of plant tissue culture. Background technique [0002] The hybrid orchid is an excellent hybrid offspring obtained from the interspecific hybridization of Cymbidium and Cymbidium. Compared with the parent, it has obvious advantages in terms of ornamental, adaptability, production and economic benefits. The hybrid orchid not only inherits the elegant posture and rich fragrance of the Chinese orchid, but also has the advantages of bright colors, large flowers, and a large number of flowers of the Cymbidium. The hybrid orchid has stronger adaptability, can oversummer in flat land, is easy to plant and maintain, and has better economic benefits. Therefore, hybrid orchids have developed rapidly in the orchid industry at home and abroad in recent years. However, the hybrid orchid varieties currently c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001A01H4/005
Inventor 李春楠傅巧娟沈国正赵福康张晓莹
Owner HANGZHOU ACAD OF AGRI SCI
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