The invention discloses a preparation method of probiotics-fermented Chinese wolfberry concentrated slurry. The preparation method comprises the following steps of crushing fresh Chinese wolfberry, dried Chinese wolfberry and Chinese wolfberry powder, so as to obtain Chinese wolfberry slurry, wherein the content of soluble solids is controlled to 15 to 30%; treating by ultrasonic waves, standing and hydrolyzing by enzyme, sterilizing, and inoculating with probiotics; warming at the temperature of 20 to 45 DEG C, and fermenting; naturally stopping fermenting, or finishing fermenting when the pH(potential of hydrogen) value reaches 3.0 to 4.5, so as to obtain probiotics-fermented Chinese wolfberry raw juice; separating the probiotics-fermented Chinese wolfberry raw juice, so as to obtain probiotics-fermented Chinese wolfberry paste; mixing 10 to 50% of probiotics-fermented Chinese wolfberry raw juice and 0 to 10% of probiotics-fermented Chinese wolfberry paste, and adding a proper amount of honey, syrup or white granulated sugar, stabilizer, sodium citrate, essence, sodium erythorbate, oligosaccharide, lutein, taurine, zinc gluconate, Vc (vitamin c) and other nutrition enhancers, soas to obtain the probiotics-fermented Chinese wolfberry concentrated slurry. The obtained probiotics-fermented Chinese wolfberry concentrated slurry has the advantages that the rich nutrients are contained, the color is bright and attractable, the flavor is elegant, and the bioactivity is higher.