New process for producing three-level strain of edible fungus

A technology of three-level strains and edible fungi, applied in the fields of horticulture, application, and botanical equipment and methods, can solve the problems of increasing labor costs, prolonging the production cycle of strains, and increasing the infection of miscellaneous bacteria.

Inactive Publication Date: 2010-02-17
天津市林业果树研究所
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Problems solved by technology

[0003] The above current process requires a lot of manpower to complete because it is bagged and sterilized first, and then inoculated. In this way, firstly, it increases the labor cost, secondly, it increases the probability of infection with miscellaneous bacteria, and thirdly, there are too few inoculation points. , leading to prolongation of the production cycle of the strain, and the process is also very complicated

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment 2

[0026] The formula of shiitake mushroom material is: 78 kg of cottonseed husk, 20 kg of bran, 1 kg of gypsum, 1 kg of calcium carbonate, and 115 kg of water. Put the solid raw materials into the mixer, close the valve, pre-mix the raw materials for 5 minutes, then add water, stir for 10 minutes, after mixing, pile up for 40 minutes, then transfer to the sterilizer, heat the compost with steam When the temperature reaches 100°C, maintain the pressure for 12 hours, stop the fire and steam for 3 hours, introduce cooling water for pipeline cooling, and inoculate when the center temperature of the compost is cooled below 25°C.

[0027] Strain treatment: Put 20 kg of the original seed of shiitake mushrooms in a sterile room, remove the bottle, crush the fungus sticks to 0.5cm-sized particles, and place them in the inoculation container.

[0028] Inoculation: Put the treated bacteria into the cultivation material through the bacteria container, stir for 10 minutes and mix well, and t...

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Abstract

The invention relates to a method for effectively preparing a three-level strain of an edible fungus, which comprises the following steps: mixing culture substrates according to a certain proportion,sterilizing, vaccinating and respectively packaging in culture containers; culturing; and obtaining finished products. The invention simplifies the process, saves a plurality of manpower, shortens theproduction period of the strain by mixing vaccination, reduces the probability of infecting mixed bacteria and realizes the aims of rapidly and efficiently manufacturing the strain of the edible fungus.

Description

technical field [0001] The invention relates to a method for cultivating and propagating edible fungus mycelia in large quantities through aseptic operation. Background technique [0002] In the actual production of edible fungi, according to the source, reproduction algebra and production purpose of the strains, the strains are divided into primary strains, secondary strains and tertiary strains. The production of edible fungus strains is completed in the order of primary strains, secondary strains, and tertiary strains. The first-class strains are generally pure cultures obtained by spore separation or tissue separation, and transplanted on a test tube slant culture medium. The first-class strains are multiplied again on the slant medium, and the obtained strains are regenerated first-class strains. The secondary strain is transplanted from the primary strain or the regenerated primary strain into a strain bottle filled with solid medium such as sawdust or dung, and is c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 张志军刘建华周永斌刘连强魏雪生訾惠君张瑾李淑芳王玫王永庄
Owner 天津市林业果树研究所
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