Preparation method of provenance spore microbial agent used in Aspergillus niger koji making process

A spore inoculum and a technology for producing Aspergillus niger, which is applied in the field of preparation of provenance spore inoculum in the process of koji making by Aspergillus niger, can solve the problems of quality fluctuation of bran koji, large production volume of eggplant bottles, complicated inoculation operation and the like, and achieve inoculation The link is simplified, the chance of contamination is reduced, and the effect of less chance of contamination

Active Publication Date: 2018-08-17
JIANGSU GUOXIN UNION ENERGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this inoculation method is that the production of eggplant bottles is relatively large, the inoculation operation is cumbersome, and the number and quality of spores scraped from each eggplant bottle will have certain differences, which will cause certain fluctuations in the quality of bran.

Method used

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  • Preparation method of provenance spore microbial agent used in Aspergillus niger koji making process

Examples

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Embodiment 1

[0032] A preparation method of provenance spore bacterial agent in the Aspergillus niger koji-making process, described method comprises the steps:

[0033] (1) Cultivate provenance spores in corncob culture medium;

[0034] The selection of corn cob carrier and the preparation of soybean meal hydrolyzate are as mentioned above, and the preparation of nutrient solution is as follows: take 800g of corn steep liquor powder and glucose, 120g of ammonium sulfate, mix and dissolve with water, and make up to 6L after dissolution. Mix 6L of nutrient solution evenly into 8kg of corncobs, and after the corncobs have completely absorbed the nutrient solution, dry them in a low-temperature drying oven at a temperature not exceeding 60°C. After drying, it is the finished culture medium.

[0035] Take four 2000ml triangular flasks, fill each bottle with 40g of corncob medium, add 60ml of water, sterilize at 121°C for 30min, cool to 35°C after sterilization, pick a ring of spores with an i...

Embodiment 2

[0043] A preparation method of provenance spore bacterial agent in the Aspergillus niger koji-making process, described method comprises the steps:

[0044] (1) Cultivate provenance spores in corncob culture medium;

[0045] The selection of corn cob carrier and the preparation of soybean meal hydrolyzate are as mentioned above, and the preparation of nutrient solution is as follows: Take 200g of corn steep liquor powder and glucose, 60g of ammonium sulfate, 3.4L of soybean meal hydrolyzate, mix and dissolve with water, and make up to 6L after dissolving. Mix 6L of nutrient solution evenly into 8kg of corncobs, and after the corncobs have completely absorbed the nutrient solution, dry them in a low-temperature drying oven at a temperature not exceeding 60°C. After drying, it is the finished culture medium.

[0046]Take four 1000ml triangular flasks, fill each bottle with 40g of corn cob medium, add 50ml of water, sterilize at 121°C for 30min, cool to 35°C after sterilization,...

Embodiment 3

[0054] A preparation method of provenance spore bacterial agent in the Aspergillus niger koji-making process, described method comprises the steps:

[0055] (1) Cultivate provenance spores in corncob culture medium;

[0056] The selection of corn cob carrier and the preparation of soybean meal hydrolyzate are as mentioned above, and the preparation of nutrient solution is as follows: take 400g of corn steep liquor powder and glucose, 120g of ammonium sulfate, 1L of soybean meal hydrolyzate, mix and dissolve with water, and make up to 6L after dissolving. Mix 6L of nutrient solution evenly into 8kg of corncobs, and after the corncobs have completely absorbed the nutrient solution, dry them in a low-temperature drying oven at a temperature not exceeding 60°C. After drying, it is the finished culture medium.

[0057] Take three 2000ml triangular flasks, fill each bottle with 60g of corn cob medium, add 84ml of water, sterilize at 121°C for 30min, cool to 35°C after sterilization...

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Abstract

The invention discloses a preparation method of a provenance spore microbial agent used in an Aspergillus niger koji making process. The method includes the steps of firstly, culturing provenance spores in a cob culture medium; secondly, preparing the provenance spore microbial agent by using a carrier adsorption separation method; thirdly, inoculating a bran koji culture container with the prepared provenance spore microbial agent to culture and prepare koji. The method has the advantages that the problems of limitation and instability of eggplant-shaped bottle provenance are solved, provenance production quantity is lowered greatly, inoculation operation steps during koji making are reduced, contamination is avoided, enterprise manpower cost is reduced greatly, and good economic benefitsare created for enterprises.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a preparation method of provenance spore bacterial agent in the process of Aspergillus niger koji making. Background technique [0002] The preparation of bran koji is the basis of citric acid production. At present, there are two main koji-making methods for citric acid production. One is to use bran to cultivate in a glass conical flask. The method is to wash the bran with water to remove the impurities in the bran. Flour, remove part of the water in the bran, so that the bran contains about 50% water, loosely pack it into a 2000ml triangular bottle, about 35 grams per bottle, put it into a ring eggplant bottle after sterilization, and cultivate Aspergillus niger spores 7 -10 days, each eggplant bottle can be connected with 50-60 triangular flasks, and it will be used after being cultivated and matured and passing the sampling inspection. When in use, about 150 ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N3/00C12M1/24
CPCC12M23/08C12N3/00
Inventor 高金宝刘振强孙士健裴云朱剑飞李小晔高超蒋小东
Owner JIANGSU GUOXIN UNION ENERGY CO LTD
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