Preparation of provenance spore microbial inoculum in starter-making process of aspergillus niger and starter-making method

A spore inoculum and a technology for producing Aspergillus niger, which are applied to the preparation of provenance spore inoculants and the field of koji making in the process of koji making by Aspergillus niger, can solve the problems of cumbersome inoculation operation, fluctuation of bran koji quality, and large amount of eggplant bottles produced. , to achieve the effect of less chance of contamination, simplifying the inoculation process, and reducing the chance of contamination

Inactive Publication Date: 2018-07-31
JIANGSU GUOXIN UNION ENERGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this inoculation method is that the production of eggplant bottles is relatively large, the inoculation operation is cumbersome, and the number and quality of spores scraped from each eggplant bottle will have certain differences, which will cause certain fluctuations in the quality of bran.

Method used

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  • Preparation of provenance spore microbial inoculum in starter-making process of aspergillus niger and starter-making method

Examples

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Effect test

Embodiment 1

[0030] A method for preparing seed-derived spores in the process of koji making by Aspergillus niger and koji making, the method includes the following steps:

[0031] (1) Cultivate seed spores in corncob culture medium;

[0032] The selection of the corncob carrier and the preparation of the soybean meal hydrolysate are as described above, and the nutrient solution is prepared as follows: Take 800g of corn steep liquor and glucose, 120g of ammonium sulfate, add water and mix to dissolve, and after dissolving, the fixed volume is 6L. Mix 6L nutrient solution evenly into 8kg corncob, and after the corncob absorbs the nutrient solution completely, place it in a low-temperature drying box for drying at a temperature not exceeding 60°C. After drying, it is the finished culture medium.

[0033] Take 4 2000ml Erlenmeyer flasks, fill each flask with 66g corncob culture medium, add 80ml of water, sterilize at 121℃ for 30min, cool to 35℃ after sterilization, pick a loop of spores with an ino...

Embodiment 2

[0040] A method for preparing seed-derived spores in the process of koji making by Aspergillus niger and koji making, the method includes the following steps:

[0041] (1) Cultivate seed spores in corncob culture medium;

[0042] The selection of the corncob carrier and the preparation of the soybean meal hydrolysate are as described above, and the nutrient solution is prepared as follows; take 200g of corn steep liquor and glucose, 60g of ammonium sulfate, 3.4L of soybean meal hydrolysate, add water and mix to dissolve, after dissolving, the fixed volume is 6L. Mix 6L nutrient solution evenly into 8kg corncob, and after the corncob absorbs the nutrient solution completely, place it in a low-temperature drying box for drying at a temperature not exceeding 60°C. After drying, it is the finished culture medium.

[0043] Take 4 2000ml Erlenmeyer flasks, fill each flask with 66g corncob culture medium, add 90ml water, sterilize at 121°C for 30min, cool to 35°C after sterilization, pick ...

Embodiment 3

[0050] A method for preparing seed-derived spores in the process of koji making by Aspergillus niger and koji making, the method includes the following steps:

[0051] (1) Cultivate seed spores in corncob culture medium;

[0052] The selection of the corncob carrier and the preparation of the soybean meal hydrolysate are as described above, and the nutrient solution is prepared as follows: Take 400g of corn steep liquor and glucose, 120g of ammonium sulfate, 1L of soybean meal hydrolysate, mix and dissolve with water, and dissolve it to a constant volume of 6L. Mix 6L nutrient solution evenly into 8kg corncob, and after the corncob absorbs the nutrient solution completely, place it in a low-temperature drying box for drying at a temperature not exceeding 60°C. After drying, it is the finished culture medium.

[0053] Take 4 2000ml Erlenmeyer flasks, fill each bottle with 80g corncob culture medium, add 96ml water, sterilize at 121℃ for 30min, cool to 35℃ after sterilization, pick a ...

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Abstract

The invention discloses preparation of provenance spore microbial inoculum in starter-making process of aspergillus niger and a starter-making method. The method comprises the steps that (1), provenance spores are cultured in a corncob culture medium; (2) the provenance spore microbial inoculum is prepared by adopting a cyclone separating method; (3), the provenance spore microbial inoculum prepared in step (2) is put into a mouldy bran culturing container to conduct starter making. By means of the method, the limitation and the unstability of eggplant-shaped bottle provenance are solved, thepreparation amount of the provenance is greatly reduced to reduce the operation procedures of inoculation in the starter-making process, the occurrence of the situation of microbiological contamination, manpower costs of enterprises are greatly saved, and better economic benefits are created for the enterprises.

Description

Technical field [0001] The present invention relates to the technical field of microbial fermentation, in particular to a preparation method of seed spore inoculum in the process of koji making by Aspergillus niger and a koji making method. Background technique [0002] The preparation of bran koji is the basis for the production of citric acid. At present, there are two main methods for preparing koji for the production of citric acid. One is to use bran to culture in a glass conical flask. The method is to wash the bran with water to remove the content of the bran. Flour, remove part of the water in the bran, make the bran about 50% water, loosely put it into a 2000ml triangular flask, each about 35 grams, after sterilization, insert the Aspergillus niger spores in a ring of eggplant bottles for cultivation 7 Within -10 days, each eggplant bottle can receive 50-60 triangular flasks, and use it after the maturity of the culture is qualified. When using, each seed tank needs to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P7/48C12R1/685
CPCC12N1/14C12P7/48
Inventor 高金宝刘振强孙士健裴云朱剑飞高超李小晔蒋小东
Owner JIANGSU GUOXIN UNION ENERGY CO LTD
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