A kind of preparation method of Aspergillus niger provenance spore agent
A spore inoculum and Aspergillus niger technology are applied in the field of preparation of provenance spore inoculum in the process of making koji, can solve the problems of quality fluctuation of bran koji, large production volume of eggplant bottles, complicated inoculation operation and the like, and achieve the inoculation link. The effect of simplifying, reducing the chance of contamination, and reducing the chance of contamination
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Embodiment 1
[0031] A kind of preparation method of aspergillus niger provenance spore agent, described method comprises the steps:
[0032] (1) Cultivate provenance spores in corncob culture medium;
[0033] The selection of corn cob carrier and the preparation of soybean meal hydrolyzate are as mentioned above, and the preparation of nutrient solution is as follows: take 800g of corn steep liquor powder and glucose, 120g of ammonium sulfate, mix and dissolve with water, and make up to 6L after dissolution. Mix 6L of nutrient solution evenly into 8kg of corncobs, and after the corncobs have completely absorbed the nutrient solution, dry them in a low-temperature drying oven at a temperature not exceeding 60°C. After drying, it is the finished culture medium.
[0034] Take two 2000ml triangular flasks, fill each bottle with 80g of corncob medium, add 128ml of water, sterilize at 121°C for 30min, cool to 35°C after sterilization, pick a ring of spores with an inoculation needle, and place ...
Embodiment 2
[0041] A kind of preparation method of aspergillus niger provenance spore agent, described method comprises the steps:
[0042] (1) Cultivate provenance spores in corncob culture medium;
[0043] The selection of corn cob carrier and the preparation of soybean meal hydrolyzate are as mentioned above, and the preparation of nutrient solution is as follows: Take 200g of corn steep liquor powder and glucose, 60g of ammonium sulfate, 3.4L of soybean meal hydrolyzate, mix and dissolve with water, and make up to 6L after dissolving. Mix 6L of nutrient solution evenly into 8kg of corncobs, and after the corncobs have completely absorbed the nutrient solution, dry them in a low-temperature drying oven at a temperature not exceeding 60°C. After drying, it is the finished culture medium.
[0044] Take two 2000ml triangular flasks, fill each bottle with 100g of corncob medium, add 120ml of water, sterilize at 121°C for 30min, cool to 35°C after sterilization, pick a ring of spores with ...
Embodiment 3
[0051] A kind of preparation method of aspergillus niger provenance spore agent, described method comprises the steps:
[0052] (1) Cultivate provenance spores in corncob culture medium;
[0053] The selection of corn cob carrier and the preparation of soybean meal hydrolyzate are as mentioned above, and the preparation of nutrient solution is as follows: take 400g of corn steep liquor powder and glucose, 120g of ammonium sulfate, 1L of soybean meal hydrolyzate, mix and dissolve with water, and make up to 6L after dissolving. Mix 6L of nutrient solution evenly into 8kg of corncobs, and after the corncobs have completely absorbed the nutrient solution, dry them in a low-temperature drying oven at a temperature not exceeding 60°C. After drying, it is the finished culture medium.
[0054] Take two 2000ml triangular flasks, fill each bottle with 66g of corn cob medium, add 100ml of water, sterilize at 121°C for 30min, cool to 35°C after sterilization, pick a ring of spores with a...
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