Culture method for inducing cherry tomato anthers to obtain regenerated plants

A technology for regenerating plants and cherry tomatoes, which is applied in the field of plant tissue culture and agricultural science, can solve the problems of not necessarily applicable, and achieve the effects of fewer steps, high practical value, and improved induction efficiency

Inactive Publication Date: 2018-12-28
江苏高航农业科技有限公司
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

This determines that the existing successful experience can be used for reference, but not necessarily applicable

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  • Culture method for inducing cherry tomato anthers to obtain regenerated plants
  • Culture method for inducing cherry tomato anthers to obtain regenerated plants

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Embodiment 1

[0031] (1) Flower buds

[0032] From April to May 2016, three varieties of cherry tomatoes 'Shengtao T6', 'Red Millennium' and 'Golden Queen' were selected as test materials (from the agricultural demonstration base of Lingshui County, Hainan Province). From 9:00 to 11:00 in the morning, collect the flower buds with a length of 0.7-0.9cm, bring them back to the laboratory in an ice box, pick 2~4 anthers from the collected flower buds, put them on a glass slide, crush them, release The pollen is produced, and the pollen development period is identified by microscopic examination with acetic acid magenta staining method, and the flower buds with pollen microspores in the single-nucleus marginal stage are screened out for later use;

[0033] (2) Bud pretreatment

[0034] Put the above-screened flower buds in a closed container at 4°C for 2 days, and then carry out surface disinfection. The disinfection method is: first clean the flower buds with washing powder, rinse them with r...

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Abstract

The invention provides a culture method for inducing cherry tomato anthers to obtain regenerated plants. The method comprises the steps as follows: (1) selection of flower buds; (2) pretreatment of flower buds; (3) stress treatment of the flower buds; (4) induced culture of flower bud embryoid; (5) seedling strengthening and rooting culture. The embryoid is directly induced through in-vitro culture of anthers, regenerated plants are obtained through seedling strengthening and rooting culture, as compared with a conventional anther culture method that dedifferentiation is performed to form callus, then, the callus is differentiated into adventitious buds and finally regenerated plants are obtained, the method is more convenient and feasible and can avoid chromosome ploidy mixing due to participation of somatic cells in formation of callus. Microspores in anthers can be induced to develop into complete haploid or double haploid plants, multiple homozygous breeding materials can be provided for haploid breeding of cherry tomatoes, the breeding efficiency is greatly improved, the breeding process is accelerated, and the method has higher application value.

Description

technical field [0001] The invention relates to a cultivation method for inducing cherry tomato anthers to obtain regenerated plants, in particular to a method for inducing embryoid bodies using cherry tomato anthers as explants, the embryoid bodies grow into young shoots, and then undergo strong seedling rooting culture to obtain single A method for regenerating ploid or double haploid plants belongs to the field of plant tissue culture and agricultural science and technology. Background technique [0002] Cherry tomato, also known as cherry tomato, small tomato, grape tomato, etc., is an annual herb plant and is the original variety of the genus Tomato in the family Solanaceae. Cherry tomato is native to the tropics. Its plant can reach a height of 2 meters. The fruit is brightly colored, with red, yellow, green and other fruit colors. Red is the most common, and its fruit shape is generally oval. Cherry tomato fruit is small in diameter, rich in water, sweet in taste, se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 王启迪
Owner 江苏高航农业科技有限公司
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