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Method for extracting triterpenoids from Scherichia cheifera leaves by using ionic liquid

A technology of ionic liquid and Liriodendron chinensis, which is applied in the fields of steroids, organic chemistry, etc., can solve problems such as extraction of triterpenoids that have not yet been seen

Active Publication Date: 2021-04-20
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, it has been reported in the literature that ionic liquids are used in the extraction of active compounds of traditional Chinese medicines. At present, there is no report on the use of ionic liquids to extract triterpenoids from Scherichia safflower leaves

Method used

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  • Method for extracting triterpenoids from Scherichia cheifera leaves by using ionic liquid
  • Method for extracting triterpenoids from Scherichia cheifera leaves by using ionic liquid
  • Method for extracting triterpenoids from Scherichia cheifera leaves by using ionic liquid

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Experimental program
Comparison scheme
Effect test

preparation example Construction

[0034] 2.1.1 Preparation of reference solution.

[0035] Accurately weigh appropriate amount of compound A and compound B, add 70% ethanol to dissolve, and make mixed reference solution with concentrations of 7.068 mg / mL and 0.49 mg / mL, respectively.

[0036] 2.1.2 Preparation of the test solution.

[0037] Accurately weigh 50.00 mg of the sample powder of Scherichia safflower leaves (passed through a 60-mesh sieve), and add 1 ml of extractant (the extractant is composed of ionic liquid [BMIM] BF 4 mixed with 70% ethanol, the concentration of ionic liquid in the extractant is 0.8 mol / L), ultrasonically extracted for 40 min, centrifuged at 6000 r / min for 5 min, and the supernatant was absorbed to obtain the test solution (in the test, the single Variable extraction agent type, extraction concentration, solid-to-liquid ratio, crushing mesh, ultrasonic extraction time, centrifugal speed, each experiment was paralleled 3 times).

[0038] 2.1.3 Chromatographic conditions and syst...

Embodiment 1

[0084] A method for extracting two main triterpenoids from Scherichia safflower leaves using ionic liquids, which mixed 50.00mg of dried Scherichia safflower leaf sample powder (60 mesh) with 1mL extractant (the extractant was prepared by ionic liquid [BMIM] BF 4 Mixed with 70% ethanol, the ionic liquid in the extractant [BMIM] BF 4 The concentration is 0.8mol / L. ) after mixing, ultrasonically extract at room temperature for 40 min, after ultrasonic extraction, centrifuge at 6000 r / min for 3 min, and take the supernatant.

[0085] After testing, it can be seen that the total content of compound A and compound B in the supernatant is 288.03 mg / g (n=3).

Embodiment 2

[0087] A method for extracting two main triterpenoids from the leaves of Scherichia safflower using ionic liquids, which mixed 50.00 mg of dried Scherichia safflower leaves (60 mesh) with 1 mL of extractant (the extractant consisted of ionic liquid [ BMIM]Br was mixed with 70% ethanol, and the concentration of ionic liquid [BMIM]Br in the extractant was 0.8mol / L.) After mixing, ultrasonically extracted at room temperature for 40 min, and after ultrasonic extraction, centrifuged at 6000 r / min for 3 min, Get the supernatant.

[0088] It can be seen from the test that the total content of compound A and compound B in the supernatant is 222.86 mg / g (n=3).

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Abstract

The invention relates to a process for optimizing the extraction process of lupine-type pentacyclic triterpenes in Scherichia chinensis leaves by ionic liquid extraction. The dry Scherichia safflower leaf powder is mixed with an extractant and then ultrasonically extracted at room temperature, and the ultrasonic extraction is completed. Afterwards, centrifuge and take the supernatant; the extractant is made of ionic liquid ([BMIM] BF 4 , [BMIM] Br, [BMIM] PF 6 or [HMIM] PF 6 ) mixed with 70% ethanol. It has been verified by experiments that compound A (3α-hydroxy-lupine-20(29)-ene-23,28-dioic acid) and compound B (3α-hydroxy-lupine-20(29)-ene-23, 28‑diacid 28‑O‑α‑L‑rhamnosyl‑(1→4)‑O‑β‑D‑glucosyl‑(1→6)‑β‑D‑glucoside) with a total content of 288.03 mg / g (n=6). Compared with the traditional extraction method, the total extraction rate of the two compounds in Scherichia safflower leaves can reach 28.80% after adding ionic liquid.

Description

technical field [0001] The invention belongs to the technical field of medicine or / and health products, and in particular relates to a method of extracting two main triterpenoids (compound A 3α-hydroxyl-lupin-20(29) -ene-23,28-dioic acid and compound B 3α-hydroxy-lupin-20(29)-ene-23,28-dioic acid 28-O-α-L-rhamnosyl-(1→4)- O-β-D-glucosyl-(1→6)-β-D-glucoside) method. Background technique [0002] Goosefoot Chai ( Schefflera octophylla (Lour.) Harms) is Araliaceae Scherichia genus, mainly distributed in tropical and subtropical regions. In China, Scherichia spp. is mainly distributed in Fujian, Guangdong, Guangxi, Yunnan and other places. Medicinal use was first recorded in "Lingnan Herbal Medicine", "Nature of Raw Herbs", "Lingnan Herb Collection" and Guangzhou Army's "Handbook of Commonly Used Chinese Herbal Medicine". Rheumatic ostalgia, bruises, stasis, swelling and pain. "Lu Chuan Materia Medica": repel bone to stop bleeding, reduce swelling and relieve pain, treat rh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J63/00
CPCC07J63/008
Inventor 康文艺许瑞安牛云刘绪强
Owner HENAN UNIVERSITY
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