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Method for extracting active components from Ganoderma lucidum assisted by ionic liquid and ultrasound

An ultrasonic-assisted extraction and ionic liquid technology, applied in the field of medicine, can solve problems such as unreported triterpenoid components, and achieve the effects of improving outdated processes and improving efficiency

Active Publication Date: 2021-08-27
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Literature survey found that the use of ionic liquids to assist the extraction of triterpenoids in Ganoderma lucidum has not been reported

Method used

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  • Method for extracting active components from Ganoderma lucidum assisted by ionic liquid and ultrasound
  • Method for extracting active components from Ganoderma lucidum assisted by ionic liquid and ultrasound
  • Method for extracting active components from Ganoderma lucidum assisted by ionic liquid and ultrasound

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Experimental program
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Effect test

preparation example Construction

[0043] 2.1.1 Preparation of reference solution

[0044] Take appropriate amount of Ganoderma acid A and Ganoderma acid D, weigh them accurately, add methanol to dissolve, and prepare mixed standard solutions with concentrations of 0.62 and 0.218 mg / mL respectively.

[0045] 2.1.2 Preparation of the test solution

[0046] Accurately weigh an appropriate amount of Ganoderma lucidum sample powder, add an appropriate amount of extracting solution according to a certain solid-liquid ratio, centrifuge after ultrasonication for a certain period of time, and absorb the supernatant to obtain the test solution (in the test, the concentration of the single variable extractant, the ultrasonic power , solid-liquid ratio, ultrasonic time, centrifugal speed, each experiment was paralleled 3 times).

[0047] 2.1.3 Chromatographic conditions and system suitability experiment

[0048] Table 1 Chromatographic conditions for determination of Ganoderma lucidum content

[0049]

[0050] Accor...

Embodiment 1

[0115] Weigh 30 mg of Ganoderma lucidum sample (20 mesh), add it to 1 mL of extractant, conduct ultrasonic extraction at room temperature at 400 W for 20 min, centrifuge at 4000 r / min after ultrasonic extraction, and take the supernatant; the extractant is composed of ionic liquid and methanol , the ionic liquid is [HMIM]Br, and the concentration of the ionic liquid in the extractant is 1.4mol / L.

[0116] It can be known from the test that the total extraction amount of the two target components in the supernatant is 3.31 mg / g (n=3).

Embodiment 2

[0118] Weigh 30 mg of Ganoderma lucidum sample (20 mesh), add it to 0.6 mL of extractant, conduct ultrasonic extraction at room temperature at 400 W for 20 min, after ultrasonic extraction is completed, centrifuge at 3000 r / min, and take the supernatant; the extractant is obtained by mixing ionic liquid and methanol Composition, the ionic liquid is [HMIM]Br, and the concentration of the ionic liquid in the extractant is 1.2mol / L.

[0119] It can be known from the test that the total extraction amount of the two target components in the supernatant is 2.78 mg / g (n=3).

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Abstract

The invention discloses a method for extracting two active ingredients of ganoderma acid A and ganoderma acid D in Ganoderma lucidum by using ionic liquid combined with orthogonal design and BP neural network optimization and predicting the extraction amount. The total extraction amount is used as an index and the ionic liquid is used. Combined with the orthogonal design for extraction investigation, the optimal extraction process of the two active components in Ganoderma lucidum was obtained: ionic liquid concentration 1.4 mol / L, ultrasonic power 400W, ultrasonic time 20 min, centrifugal speed 4000r / min, solid-liquid ratio 1 g: 33.3 mL. Under these conditions, the extraction amount of active ingredients in Ganoderma lucidum was 3.31 mg / g ( n =3). The results show that under the considered experimental conditions, the neural network model can effectively simulate the experimental data and reproduce the process behavior, and the use of the BP neural network model can predict the total amount of target compound extraction with low energy consumption, high efficiency, and accuracy, which will provide a basis for the development of Ganoderma lucidum The application provides a scientific basis.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a method for extracting ganoderma acid A and ganoderma acid D in ganoderma lucidum by using ionic liquid and ultrasonic auxiliary extraction (orthogonal design and BP neural network optimization extraction) and predicting the extraction amount. Background technique [0002] The traditional Chinese medicine Ganoderma lucidum is the dry fruiting body of Ganoderma lucidum (Leyss.exFr.) Karst or Ganoderma sinense Zhao, Xu et Zhang of Polyporaceae. It has a medicinal history of more than two thousand years. The Chinese Pharmacopoeia records: sweet in taste, flat in nature, homecoming to the heart, lung, liver, and kidney meridian, has the functions of invigorating qi and calming the nerves, relieving cough and relieving asthma. [0003] Studies have shown that Ganoderma lucidum has pharmacological activities such as immune regulation, anti-tumor, liver protection, hypogly...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01D11/02G01N30/02G01N30/06G01N30/14
CPCB01D11/0265B01D11/02G01N30/02G01N30/06G01N30/14G01N2030/062G06N3/084G06N3/045
Inventor 李昌勤王金梅刘振花崔丽丽康文艺崔一平梁振华卢洁
Owner HENAN UNIVERSITY
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