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Method for extracting phloridzin, astragalin and afzelin from apple blossom by utilizing ionic liquid

A technology of ionic liquid and astragaloside, applied in the field of medicine or/and health care products, can solve problems such as no reports on ionic liquid extraction research, and achieve the effects of improving old processes, high extraction efficiency and improving efficiency

Active Publication Date: 2018-08-14
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, it has been reported in the literature that ionic liquids are used in the extraction of active compounds of traditional Chinese medicines, but there is no report on the extraction of flavonoids in apple flowers by ionic liquids.

Method used

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  • Method for extracting phloridzin, astragalin and afzelin from apple blossom by utilizing ionic liquid
  • Method for extracting phloridzin, astragalin and afzelin from apple blossom by utilizing ionic liquid
  • Method for extracting phloridzin, astragalin and afzelin from apple blossom by utilizing ionic liquid

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Experimental program
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preparation example Construction

[0033] 2.1.1 Preparation of reference solution

[0034] Take appropriate amount of phlorizin, astragalin and afodouside, weigh them accurately, add methanol to dissolve, and prepare mixed reference substance solutions with concentrations of 0.204, 0.196 and 0.199 mg / mL, respectively.

[0035] 2.1.2 Preparation of the test solution

[0036] 10 mg of apple blossom sample powder (70 mesh) was precisely weighed, and an extractant was added (the extractant was composed of ionic liquid [BMIM]Br mixed with methanol, and the concentration of ionic liquid [BMIM]Br in the extractant was 0.52 mol / L. ) 1 mL, after ultrasonic extraction at room temperature for 60 min, centrifuge at 7000 r / min for 3 min, absorb the supernatant, filter through a 0.22 μm microporous membrane, take the filtrate, and obtain the test solution (the above-mentioned crushing mesh, extraction agent , solid-liquid ratio, ultrasonic time and centrifugation speed are the optimal conditions screened out).

[0037]2.1....

Embodiment 1

[0090] A method for extracting phlorizin, ascarin and afosidin from apple blossoms using ionic liquids, which mixed 0.5 g of dried apple blossom powder (50 mesh) with 20 mL of extractant (the extractant consisted of ion Liquid [BMIM] BF 4 Mixed with methanol, the ionic liquid in the extractant [BMIM] BF 4 The concentration is 0.7 mol / L. ) were mixed, then ultrasonically extracted at room temperature for 30 min, after the ultrasonic extraction was completed, centrifuged at 3000 r / min for 3 min, and the supernatant was obtained.

[0091] It was detected that: the total content of phloridin, ascarin and afodouside in the supernatant was 88.54 mg / g (n=3).

Embodiment 2

[0093] A method for extracting phlorizin, ascarin and afosidin from apple blossoms using ionic liquids, which mixed 0.5 g of dried apple blossom powder (50 mesh) with 20 mL of extractant (the extractant consisted of ion Liquid [BMIM]Br mixed with methanol, the concentration of ionic liquid [BMIM]Br in the extractant is 0.7 mol / L.) After mixing, ultrasonically extract at room temperature for 30 min, after ultrasonic extraction, centrifuge at 3000 r / min for 3 min, Get the supernatant.

[0094] It can be seen from the test that the total content of phlorizin, ascarin and afodouside in the supernatant is 99.98 mg / g (n=3).

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Abstract

The invention relates to a method for extracting phloridzin, astragalin and afzelin from apple blossom by utilizing ionic liquid. The method comprises the following steps: mixing dried apple blossom powder with an extraction agent and then carrying out ultrasonic extraction at room temperature; after the ultrasonic extraction is finished, centrifuging and taking supernatant, thus obtaining a finished product, wherein the extraction agent is prepared by mixing the ionic liquid with methanol. The total content of the phloridzin, the astragalin and the afzelin in a verification test is 145.4mg / g.Compared with a traditional extraction method, the method has the advantage that the total extraction rate of the phloridzin, the astragalin and the afzelin in the apple blossom after the ionic liquid is added can reach 25.4 percent. The result shows that the ionic liquid is combined with a response surface method to screen an optimum extraction process in the method disclosed by the invention; in addition, an apple blossom methanol extracting solution has a certain activation effect on tyrosinase while a methanol ionic liquid extracting solution has a certain inhibitory effect on the tyrosinase. The invention provides scientific basis for development and utilization of apple blossom energy.

Description

technical field [0001] The invention belongs to the technical field of medicine and / or health products, and in particular relates to a method for extracting phlorizin, astragalin and afodouside from apple blossoms by using ionic liquid combined with response surface method. Background technique [0002] Apple flower is Rosaceae Malus plant apple Malus pumila Mill's dried flowers. The chemical components of apple blossoms mainly include dihydrochalcones such as phlorizin and phloretin, and other flavonoids such as quercetin, kaempferol, rutin, etc. Apple flower tea has the functions of nourishing blood, relieving neuralgia, liver spots, improving eyesight, treating dark spots, pimples, acne, beautifying skin, helping digestion, protecting liver and promoting blood circulation, etc. Pharmacological studies have shown that flavonoids have pharmacological effects such as anti-oxidation, anti-inflammation, analgesia, immune regulation, anti-aging, lowering blood fat, and anti-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/203C07H17/07C07H1/08A61P17/00
CPCA61P17/00C07H1/08C07H15/203C07H17/07
Inventor 康文艺李文静王金梅李昌勤王鹏禹呼谧允
Owner HENAN UNIVERSITY
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