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Method for extracting myricetrin, quercetin and afzelin from cercis chinensis leaves by utilizing ionic liquid

A technology of ionic liquid and afodouside, which is applied in the fields of medicine and/or health care products, can solve the problems of unseen extraction reports, and achieve the effects of high extraction efficiency, improvement of old technology, and simple operation

Active Publication Date: 2018-06-29
HENAN UNIVERSITY
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AI Technical Summary

Problems solved by technology

[0004] At present, there is no report on the extraction of flavonoids such as myricetin and quercetin from Bauhinia leaves by ionic liquids in literature research

Method used

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  • Method for extracting myricetrin, quercetin and afzelin from cercis chinensis leaves by utilizing ionic liquid
  • Method for extracting myricetrin, quercetin and afzelin from cercis chinensis leaves by utilizing ionic liquid
  • Method for extracting myricetrin, quercetin and afzelin from cercis chinensis leaves by utilizing ionic liquid

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Experimental program
Comparison scheme
Effect test

preparation example Construction

[0030] 2.1.1 Preparation of reference solution

[0031] Take appropriate amount of myricetin, quercitrin and afudouside, weigh them accurately, add methanol to dissolve, and prepare a mixed reference solution containing 249.86 μg myricetin, 69.58 μg quercitrin and 22.91 μg afudouside per ml.

[0032] 2.1.2 Preparation of the test solution

[0033] Accurately weigh 1 g of Bauhinia leaf powder (90 mesh), add extractant (the extractant is composed of ionic liquid [BMIM] BF 4 Mixed with 70% ethanol, the ionic liquid in the extractant [BMIM] BF 4 The concentration is 0.7 mol / L. ) 50 mL, ultrasonically extract at room temperature for 50 min, centrifuge at 3000 r / min for 5 min, absorb the supernatant, filter through a 0.22 μm microporous membrane, and take the filtrate to obtain (the above-mentioned crushing mesh, extraction solution, solid-liquid Ratio, ultrasonic time and centrifugal speed are the optimal conditions for the design factors of the orthogonal experiment).

[0034]...

Embodiment 1

[0083] A method for extracting myricetin, quercitrin and afosidin from Bauhinia leaves by using ionic liquid, which comprises 1 g of dried Bauhinia leaf powder (90 mesh) and 50 mL of extractant (the extractant is prepared by ionic liquid [BMIM] BF 4 Mixed with 70% ethanol, the ionic liquid in the extractant [BMIM] BF 4 The concentration is 0.7 mol / L. ) after mixing, ultrasonically extract at room temperature for 50 min, after ultrasonic extraction, centrifuge at 3000 r / min for 5 min, and take the supernatant.

[0084] The results showed that the contents of myricetin, quercitrin and afodouside in the supernatant were 8.6915, 1.5865 and 1.0920 mg / g, respectively (n=3).

Embodiment 2

[0086] A method for extracting myricetin, quercitrin and afosidin from Bauhinia leaves by using ionic liquid, which comprises 1 g of dried Bauhinia leaf powder (90 mesh) and 50 mL of extractant (the extractant is prepared by ionic liquid [BMIM] Br mixed with 70% ethanol, the concentration of ionic liquid [BMIM] Br in the extractant is 0.7 mol / L.) After mixing, ultrasonically extract at room temperature for 50 min, after ultrasonic extraction, centrifuge at 3000 r / min for 5 min, take the The clear liquid is ready.

[0087] The results showed that the contents of myricetin, quercitrin and afodouside in the supernatant were 4.3237, 1.0285 and 0.7819 mg / g, respectively (n=3).

[0088] Conclusion: In this experiment, ionic liquid-ultrasound-assisted extraction of flavonoids such as myricetin in Bauhinia leaves is expected to provide a theoretical basis for the development and utilization of Bauhinia leaves. However, this paper has not found the regularity between the types of ioni...

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Abstract

The invention relates to a method for extracting myricetrin, quercetin and afzelin from cercis chinensis leaves by utilizing ionic liquid. The method is characterized by comprising the following steps: mixing dried cercis chinensis leaf powder with an extraction agent, then performing ultrasonic extraction at room temperature, performing centrifugation after the ultrasonic extraction is finished,and taking supernatant liquid, wherein the extraction agent is prepared by mixing ionic liquid with ethanol. Verification tests show that the average content of myricetrin, quercetin and afzelin is respectively 8.6915mg / g, 1.5865mg / g and 1.0920 mg / g (n=3). Results show that an optimal extraction process is screened by adopting the ionic liquid combined with an orthogonal test, and a scientific basis is provided for energy development and utilization of cercis chinensis leaves.

Description

technical field [0001] The invention belongs to the technical field of medicine and / or health care products, and in particular relates to a method for optimally extracting myricetin, quercetin and afosidin from Bauhinia leaves by using an ionic liquid combined with an orthogonal test method. Background technique [0002] Bauhinia leaves are leguminous plants Bauhinia ( Cercis chinensis ) dry leaves, its chemical components mainly include flavonoids, stilbenes, phenolic acids, lignans, cyanogenic glycosides, etc. Studies have found that ethanol extracts of Bauhinia leaves and stems can scavenge 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) free radicals and inhibit Fe2 + 20 compounds including myricetin, quercetin and afudouside were separated from the ethanol extract of Bauhinia leaves by activity tracking method, among which flavonoids such as myricetin and quercetin It has antioxidant, anti-tumor and hepatoprotective activities. [0003] Therefore, choosing a suitable e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08
CPCC07H1/08C07H17/07
Inventor 李昌勤康文艺王金梅史梦珺何楠呼谧允
Owner HENAN UNIVERSITY
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