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Human red blood cell rare blood type genetic typing primer group and application

A genotyping, red blood cell technology, applied in recombinant DNA technology, microbial assay/examination, biochemical equipment and methods, etc.

Inactive Publication Date: 2019-01-04
江苏中济万泰生物医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the prior art, there is a lack of a comprehensive and holistic assay kit for human erythrocyte rare blood group genotyping

Method used

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Examples

Experimental program
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Effect test

Embodiment

[0021] A kit for detecting rare genotyping of human erythrocytes, comprising primers of SEQ ID No.1-86 sequence, dNTP-Buffer, cresyl red sodium salt and MgCl 2 . The test for each person includes 43 test holes, each of which is placed in the test holes.

[0022] Storage conditions and expiration date

[0023] All components of the kit should be stored below -20±5°C, and the validity period is 12 months. Repeated freezing and thawing should be avoided. Please refer to the label for the production date and expiration date.

[0024] Sample request:

[0025] Applicable samples: human genomic DNA;

[0026] Concentration: 40-100ng / ul; Quality: A260 / 280: 1.6-2.0.

[0027] Testing method

[0028] 1. Preparation of PCR working solution (in the reagent preparation area)

[0029] Add 500ul of deionized water and Taq enzyme (Promega 10ul or our product rTaq 12ul) into the PCR concentrate tube.

[0030] 2. Mixer 8 times at intervals of 10 seconds or (slowly upside down 10 times)

...

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PUM

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Abstract

The invention discloses a human red blood cell rare blood type genetic typing primer group. The human red blood cell rare blood type genetic typing primer group comprises 43 pairs of primers as shownin SEQ ID NO. 1-86. Specific primers are designed for 12 clinically important red blood cell antigen genes, in combination with SYBR Green I, real-time fluorescent PCR amplification and melting curvereaction are carried out, when a primer sequence and a to-be-detected sequence complete each other, a target fragment is copied and amplified through PCR reaction, and then the temperature (TM) of a specific product when double strands are dissociated by 50% is detected. An internal reference (IC) is added in a PCR system, and is used for evaluating sample quality and PCR inhibiting factors.

Description

technical field [0001] The invention relates to the technical field of gene diagnosis products, in particular to a human erythrocyte rare blood type genotyping primer set and its application. Background technique [0002] For immune erythrocyte alloantibodies in plasma or expected multiple blood transfusions, multiple erythrocyte antigen-matched blood transfusions can be used to ensure clinical blood safety. However, the prior art lacks a comprehensive and integral assay kit for genotyping human erythrocyte rare blood groups. Contents of the invention [0003] The technical problem to be solved by the present invention is to overcome the lack of a comprehensive and integral assay kit for human red blood cell rare blood genotyping in the prior art, and provide a human red blood cell rare blood genotyping primer set and its application. [0004] In order to solve the problems of the technologies described above, the present invention provides the following technical solutio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6876C12N15/11
CPCC12Q1/6876
Inventor 林裕翔吴明磊朱晓洁
Owner 江苏中济万泰生物医药有限公司
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