A canine Pediococcus lactis and its application

A technology derived from Pediococcus lactis and canine origin is applied in the field of microorganisms to achieve the effects of reducing production costs, increasing the content of lactic acid bacteria, and increasing the depth of jejunal crypts

Active Publication Date: 2021-07-20
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Excellent strains are the basis for the development of canine microecological preparations. Probiotics derived from the host itself have been proven to be safer and more effective. However, currently there are few types of probiotic strains used in the development of canine microecological preparations, and most of them come from human probiotics. Therefore, there is an urgent need to screen out safe canine-derived probiotics with probiotic functions

Method used

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  • A canine Pediococcus lactis and its application
  • A canine Pediococcus lactis and its application
  • A canine Pediococcus lactis and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Isolation, identification and safety evaluation of Pediococcus lactis R76

[0036] 1. Materials and methods

[0037] 1.1 Materials

[0038] 1.1.1 Sample source and medium preparation

[0039] (1) Sample source: The feces of a healthy unweaned Border Collie puppy were collected in Nanguan District, Changchun City.

[0040] (2) Preparation of MRS liquid medium

[0041]

[0042] Mix the reagent with 1L of distilled water, adjust the pH value to 6.8, and autoclave at 115°C for 15 minutes.

[0043] (3) MRS solid medium: Add 1.5 g of agar powder to every 100 mL of MRS liquid medium, and autoclave at 115° C. for 15 minutes.

[0044] 1.1.2 Experimental animals

[0045] 30 SPF grade BALB / c mice, 18g-22g, were purchased from Beijing Huafukang Company (Certificate No. 11401300071553).

[0046] 1.1.3 Main test reagents

[0047]

[0048]

[0049] 1.2 Method

[0050] 1.2.1 Isolation of lactic acid bacteria from fecal samples

[0051]Collect the feces of a ...

Embodiment 2

[0128] Example 2 Effect of Pediococcus lactis on intestinal morphology, flora and blood indicators of puppies

[0129] 1 Materials and methods

[0130] 1.1 Materials

[0131] 1.1.1 Strains

[0132] Pediococcus lactis R76 deposited in the present disclosure.

[0133] 1.1.2 Experimental animals and management

[0134] 20 weaned healthy puppies (60 days old) were fed with puppies food twice a day (see Table 2-1 for nutritional composition), had free access to drinking water, and cleaned the kennels every day.

[0135] Table 2-1 Nutrient composition of dog food for puppies

[0136]

[0137] 1.1.3 Experimental main reagents

[0138]

[0139] 1.2 Method

[0140] 1.2.1 Effect of Pediococcus lactis on growth performance of puppies

[0141] 20 weaned healthy puppies were randomly divided into groups, and the test group and the PBS buffer control group (grouping and oral dosage were shown in Table 2-2) were set to be fed with Pediococcus lactis preparation, fed once a day, ...

Embodiment 3

[0203] Example 3 Study on the effect of canine-derived Pediococcus lactis on alleviating the stress of transporting puppies

[0204] 1 Materials and methods

[0205] 1.1 Materials

[0206] 1.1.1 Strains

[0207] Pediococcus lactis R76 deposited in the present disclosure.

[0208] 1.1.2 Experimental animals and management

[0209] A pet shop in Changchun purchased a batch of weaned puppies. After long-distance transport by car, 20 of them developed stress reactions, mainly manifested as reduced feed intake and severe diarrhea. The control group was given PBS buffer solution (see Table 3-1 for grouping and oral dosage), fed once a day, and the transport-stressed puppies were fed the same puppy food, the kennels were cleaned every day, and drinking water was free.

[0210] Table 3-1 Puppies grouping and oral dosage form

[0211]

[0212] 1.2 Method

[0213] 1.2.1 Effect of Pediococcus lactis on recovery of feed intake in transport-stressed puppies

[0214] The puppies i...

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Abstract

This disclosure relates to a strain of canine Pediococcus acidilactici and its application. The classification of the strain is named Pediococcus acidilactici (Pediococcus acidilactici), the number is JLAPCC‑P76, and it has been preserved in the Chinese Type Culture Collection Center on July 28, 2018. , the address is: China. Wuhan. Wuhan University, and its deposit number is CCTCC M 2018500. The present disclosure takes the fresh feces of puppies as a sample, and screens out lactic acid bacteria strains with excellent characteristics from them. After continuously feeding weaned puppies for 8 weeks, the intestinal morphology, flora and blood indicators are measured to evaluate its effect on puppies. The effects of dog digestion and absorption, intestinal flora, and antioxidant properties. At the same time, in order to evaluate the therapeutic effect of the screened lactic acid bacteria on the stress symptoms of pet puppies, the disclosure gave stress puppies with loss of appetite and diarrhea after long-distance transportation. The dogs were fed with lactic acid bacteria, and their diarrhea recovery and diet recovery were observed, so as to relieve the common symptoms of transportation stress in the pet dog trading process.

Description

technical field [0001] The disclosure belongs to the technical field of microorganisms, and in particular relates to a strain of Pediococcus lactis derived from canine and its application. Background technique [0002] The statements herein merely provide background information related to the present disclosure and may not necessarily constitute prior art. [0003] Probiotics are a type of active microorganisms that can produce beneficial effects on the health of the host when the body ingests an appropriate amount. The main strains are Lactobacillus, Bacillus, Bifidobacterium, Enterococcus and Pediococcus. It is made into microecological preparation products to solve some of the problems encountered in animal feeding. At present, the number of registered dogs in my country has reached 70 million, which puts forward a higher demand for healthy dog ​​breeding. Studies have shown that when dogs use probiotics, probiotics can improve the dog's immunity, reduce stress responses...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/744A61P1/12A61P1/14C12R1/01
CPCA61K2035/115A61P1/12A61P1/14C12N1/20C12N1/205C12R2001/01
Inventor 王春凤王建忠杨桂连石春卫李宁单宝龙谷巍王红
Owner JILIN AGRICULTURAL UNIV
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