Compound microbial agent for controlling powdery mildew of Euonymus japonicus

A compound microbial inoculant, a technology of boxwood, applied in the directions of microorganisms, biocides, and methods based on microorganisms, can solve problems such as the impact of control effects, and achieve the effects of low cost, quick effect, and good treatment effect.

Inactive Publication Date: 2019-01-18
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the control effect of spraying a single biocontrol agent is not as good as that of the compound agent, and the ratio of the compound agent has a great influence on the control effect. Must do

Method used

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  • Compound microbial agent for controlling powdery mildew of Euonymus japonicus
  • Compound microbial agent for controlling powdery mildew of Euonymus japonicus

Examples

Experimental program
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Effect test

Embodiment 1

[0020] A compound microbial bacterial agent for preventing and treating powdery mildew of Euonymus japonicus comprises the steps of:

[0021] (1) Inoculate Paecilomyces lilacinus into 100 ml of PDA liquid medium, and culture it in a shaker at 25° C. and 150 rpm for 26 hours to obtain bacterial solution A;

[0022] (2) Inoculate Bacillus coliformis in 100ml of LB liquid medium, and culture it in a shaking table at 30°C and 150rpm for 26h to obtain bacterial solution B;

[0023] (3) Mix 7ml of bacterial solution A and 3ml of bacterial solution B to obtain the mixed bacterial solution, add double distilled water to 100ml, and obtain a composite microbial bacterial agent for preventing and treating powdery mildew of Euonymus euonymus.

Embodiment 2

[0025] A compound microbial bacterial agent for preventing and treating powdery mildew of Euonymus japonicus comprises the steps of:

[0026] (1) Inoculate Paecilomyces lilacinus into 100 ml of PDA liquid medium, and culture it in a shaker at 28° C. and 180 rpm for 22 hours to obtain bacterial solution A;

[0027] (2) Inoculate Bacillus coliformis in 100ml of LB liquid medium, and culture it in a shaking table at 35°C and 180rpm for 22h to obtain bacterial solution B;

[0028] (3) Mix 8ml of bacterial solution A and 2ml of bacterial solution B to obtain the mixed bacterial solution, add double distilled water to 100ml, and obtain a composite microbial bacterial agent for preventing and treating powdery mildew of Euonymus euonymus.

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Abstract

The invention discloses a compound microbial agent for controlling powdery mildew of Euonymus japonicas. The preparation method comprising the following steps: (1) Paecilomyces lilacinus is inoculatedin 100ml PDA liquid culture medium and cultured in a shaking table at 25-28 DEG C and 150-180rpm; 26h, obtain bacterial liquid A; (2) Bacillus gliofaciens is inoculated in 100ml LB liquid culture medium and cultured in shaking at 30- 35 DEG C and 150 - 180rpm. 26h, obtain bacterial liquid B; 3. Mix 7-8 parts of bacterial liquid A and 2-3 part of bacterial liquid B according to that mass ratio toobtain the mixed bacterial liquid, adding double distilled water to 100 parts of the mixed bacterial liquid, and obtaining a composite microbial agent for controlling the powdery mildew of Euonymus japonicus (L.) The method has the advantages of low cost, simplicity and rapidity, and can effectively prevent and cure the powdery mildew of Euonymus japonicus, and has quick effect and good curative effect.

Description

technical field [0001] The invention relates to the field of plant disease prevention and control, in particular to a compound microbial bacterial agent for preventing and treating powdery mildew of Euonymus euonymus. technical background [0002] Euonymus japonicus Thunb (Euonymus japonicus Thunb), also known as holly Euonymus and orthodox, belongs to Euonymus family. It is a temperate and subtropical tree. It is widely cultivated in my country and is a common hedge plant and modeling material in landscaping. It has great economic and social importance. benefit. According to reports, the occurrence of diseases of Euonymus euphratica is relatively common, among which powdery mildew of Euonymus euphratica is more serious. Euonymus powdery mildew is caused by the infection of Erysipheeuonymi-japonici, which can occur from seedling to growth stage, and mainly damages leaves and stems of Euonymus japonici, while young leaves and new shoots are most susceptible Infected by powde...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/20A01N63/00A01N63/04A01P3/00C12R1/79C12R1/07
CPCA01N63/00A01N63/30C12N1/14C12N1/20
Inventor 王罡张亚楠季静孙培
Owner TIANJIN UNIV
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