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Blood or tissue miRNA population for prognosis of colorectal cancer and use thereof

A colorectal cancer, group technology, applied in the field of biomedicine, can solve the problems of large trauma, poor patient acceptance, and cumbersome process, and achieve the effect of quickly and accurately grasping the patient's condition

Inactive Publication Date: 2019-01-18
安广宇
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

2. The detection of miRNA in tissue samples is very invasive to patients, and pathological section detection is required. The process is relatively cumbersome and the acceptance of patients is poor.

Method used

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  • Blood or tissue miRNA population for prognosis of colorectal cancer and use thereof
  • Blood or tissue miRNA population for prognosis of colorectal cancer and use thereof
  • Blood or tissue miRNA population for prognosis of colorectal cancer and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Example 1 Screening of miRNAs associated with human colorectal cancer

[0069] 1. Collection of samples and collation of sample data

[0070] Plasma samples from 110 patients with colorectal cancer, tumor tissue samples from 14 patients with colorectal cancer, 12 paracancerous tissues and 38 normal people from the health examination center were collected from the Oncology Department of Beijing Chaoyang Hospital. All patients were advanced colorectal cancer patients, TNM stage III-IV stage, receiving chemotherapy in our department.

[0071] 2. Extraction of plasma RNA

[0072] Take 500ul plasma sample of patient, extract the RNA in the plasma by miRNeasy Mini Kit (Qiagen, 217004), the method is as follows:

[0073] 1. Add 700ul QIAzol cracking solvent to the plasma sample, blow and mix with a gun;

[0074] 2. Place the above mixed solution at room temperature (15°C-25°C) for 5 minutes;

[0075] 3. Add 140ul chloroform, shake and mix for 15s;

[0076] 4. Place at roo...

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Abstract

The invention discloses a blood or tissue miRNA population for prognosis of colorectal cancer and use thereof, which quantitatively detects the miRNA population and realizes the miRNA detection of peripheral blood or tumor tissue of a patient. At first, that miRNA population in the peripheral blood or tumor tissue of the colorectal canc patient is separated and purified. The expression of miRNA incolorectal cancer patients and normal people was confirmed by QPCR. The miRNA chip can be used as a prognostic index to evaluate the prognosis of colorectal cancer patients. It can be used to preparethe chip or kit to judge the prognosis of colorectal cancer patients.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to a blood or tissue miRNA group related to colorectal cancer and its application; it is used to detect the expression of microRNA related to peripheral blood or tissue of patients, and to predict the survival period of patients, and specifically relates to the expression of miRNA in colorectal cancer. Prognostic applications in rectal cancer. Background technique [0002] Colorectal cancer is one of the common malignant tumors of the digestive tract, and its mortality rate ranks third in developed countries. In China and other developing countries, the incidence of colorectal cancer is also increasing year by year. At present, the 5-year survival rate of patients with colorectal cancer in the world can reach about 65%, but the 5-year survival rate of advanced patients is only about 10%. In recent years, with the gradual deepening of the research on colorectal cancer, we have...

Claims

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Application Information

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IPC IPC(8): C12Q1/6837C12Q1/6886C12N15/113
CPCC12Q1/6886C12Q1/6837C12Q2600/118C12Q2600/158C12Q2600/178
Inventor 葛洋
Owner 安广宇
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