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SNP molecular marker related to sheep prolificacy, and detection kit and application thereof

A technology of molecular markers and kits, which is applied in the determination/testing of microorganisms, recombinant DNA technology, biochemical equipment and methods, etc., can solve the problems that the SIX1 gene has not been discovered, and its function has not been reported, so as to improve the rate of estrus and breeding, Improve lambing performance, cost-effective effect

Active Publication Date: 2019-01-18
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no report on the specific role of SIX1 gene sequence polymorphism in the process of sheep reproduction at home and abroad, and no SNP sites related to the shape of sheep with multiple lambs have been found in the SIX1 gene

Method used

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  • SNP molecular marker related to sheep prolificacy, and detection kit and application thereof
  • SNP molecular marker related to sheep prolificacy, and detection kit and application thereof
  • SNP molecular marker related to sheep prolificacy, and detection kit and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] A way to use Sequenom SNP technology to detect sheep SIX1 genotype and predict the average litter size of multiparous ewes

[0079] 1. Experimental materials

[0080] Select seasonal estrus single-lamb breeds (Tan sheep, Sunit sheep, grassland type Tibetan sheep, a total of 204) and perennial estrus multi-lamb breeds (560 small-tailed Han sheep, Hu sheep, and Cele black sheep, including 380 small-tailed Han sheep with lambing records) were tested.

[0081] 2. Reagents and instruments

[0082] Reagent: Complete Genotyping Reagent Kit for Compact 384;

[0083] Gene Amplification: ABI 9700384 Dual;

[0084] Mass spectrometry spotting: MassARRAYNanodispenserRS1000;

[0085] Mass spectrometry: MassARRAY Compact System;

[0086] All reagents and instruments were purchased from Beijing Genenode Biotech Co., Ltd.

[0087] 3. Genomic DNA extraction

[0088] 1 mL of blood was collected from the jugular vein of the sheep and anticoagulated with EDTA. First, erythro...

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Abstract

The invention provides a SNP molecular marker related to sheep prolificacy, and a detection kit and application thereof, and belongs to sheep SNP molecular marker technology field. The SNP locus is located at the 69738971bp locus on chromosome 7 (NM_001174113.1, based on the sheep genome sequence information version number Oar_v3.1), and G / T base mutation exists on the SNP locus, which has significant correlation with lambing traits of sheep. The SNP loci can be typed to predict lambing traits of sheep to be tested. The method for detecting the genotype of the SNP locus of sheep provided by the invention has higher sensitivity, accuracy and cost performance ratio, can realize automatic detection of the SNP locus, can select and retain TT homozygous individuals with multiple lamb traits insheep breeding process, and has potential application value for sheep large-scale molecular breeding.

Description

technical field [0001] The invention belongs to the technical field of sheep SNP molecular markers, and in particular relates to a SNP molecular marker related to the trait of multiple lambs in sheep, a detection kit and application thereof. Background technique [0002] Most sheep breeds have seasonal breeding characteristics, coupled with their own low heritability of litter size traits, resulting in low numbers of sheep in the natural state. In recent years, with the increasing demand of my country's mutton sheep market, people have gradually adopted various methods to improve the production efficiency of mutton sheep, among which molecular technology can effectively improve the genetic progress of sheep. Therefore, the screening of major genes or molecular genetic markers related to lamb size has become a hot spot in modern molecular breeding. [0003] The SIX1 gene is located on the chromosome 7 of sheep, including 2 exons, the total length of the coding region is 1068...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156C12Q2600/124
Inventor 狄冉储明星李春艳刘秋月胡文萍王翔宇
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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