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A kind of cyclic fermentation method of propionibacterium

A technology of propionibacterium and cyclic fermentation, which is applied in the direction of microorganism-based methods, fermentation, biochemical equipment and methods, etc., can solve the problems of bacterial loss and other problems, and achieve the enhancement of bacterial metabolites, cost reduction, and product cost reduction Effect

Active Publication Date: 2022-03-25
ZHUMADAIN TIANZHONG BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, when the fermentation broth is released, there is still a loss of bacteria

Method used

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  • A kind of cyclic fermentation method of propionibacterium

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Embodiment 1

[0029] A method for circulating fermentation of propionibacterium, comprising the following steps:

[0030] (1) The cultured propionibacterium thalline enters the fermenter containing the culture medium for fermentation to obtain a fermented liquid;

[0031] (2) The fermented liquid in step (1) is subjected to ceramic membrane circulation filtration to obtain the primary thalline concentrated solution, the volume of the primary thalline concentrated liquid is 25% of the total volume of the fermented liquid, and an equal volume is added to the primary thalline concentrated liquid The sterile pure water, and then continue to filter through the ceramic membrane to obtain the secondary cell concentrate, the volume of the secondary cell concentrate is the same as the volume of the primary cell concentrate, and the secondary cell concentrate is sent to the fermenter In the process, fresh culture medium is supplemented for fermentation, the volume ratio of the secondary bacterium con...

Embodiment 2

[0040] This embodiment is basically the same as Example 1, except that in step (2), the fermented liquid is circulated and filtered through a ceramic membrane to obtain a primary cell concentrate, and the volume of the primary cell concentrate is 24% of the total volume of the fermentation liquid , add an equal volume of sterile pure water to the primary cell concentrate, and then continue to perform ceramic membrane circulation filtration to obtain a secondary cell concentrate, the volume of the secondary cell concentrate is the same as that of the primary cell concentrate, Send the secondary bacterial cell concentrate to the fermenter, and add new medium, the volume ratio of the secondary bacterial cell concentrated liquid to the fresh medium is 1:2.5.

[0041] The medium of fermentation tank in the step (1): calcium carbonate 12g / L, yeast extract powder 10g / L, glucose 35g / L, pH value is natural; Along with carrying out in the fermentation tank, according to the variation of ...

Embodiment 3

[0044] This embodiment is basically the same as Example 1, except that in step (2), the fermented liquid is circulated and filtered through a ceramic membrane to obtain a primary cell concentrate, and the volume of the primary cell concentrate is 26% of the total volume of the fermented liquid , add an equal volume of sterile pure water to the primary cell concentrate, and then continue to perform ceramic membrane circulation filtration to obtain a secondary cell concentrate, the volume of the secondary cell concentrate is the same as that of the primary cell concentrate, The secondary bacterial cell concentrate is sent to the fermenter, and new medium is added, and the volume ratio of the secondary bacterial cell concentrated liquid to the fresh medium is 1:3.5.

[0045] The medium of fermentor in the step (1): calcium carbonate 15g / L, yeast extract powder 18g / L, glucose 40g / L, pH value is natural; Along with carrying out in fermentor, according to the change of fermented liqu...

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Abstract

The present invention proposes a method for cyclic fermentation of propionibacterium, comprising the following steps: (1) the cultured propionibacterium thallus enters a fermentation tank containing a culture medium for fermentation to obtain a fermentation liquid; (2) the step ( 1) The fermented liquid in 1) is subjected to ceramic membrane circulation filtration, and the fermented liquid supernatant liquid containing propionic acid is obtained by filtration, and the thalline concentrate liquid containing propionibacterium is sent to the fermentation tank, and fresh culture medium is added to carry out fermentation; (3) Circulation Step (2), until the acid production rate drops significantly, stop the cycle; (4) Repeat steps (1)-(3) to start a new round of cyclic fermentation of Propionibacterium. The invention adopts ceramic membrane filtration, realizes the recycling of the propionibacterium thallus, shortens the cultivation cycle of the propionibacterium thallus, reduces the production cost, and improves the production efficiency.

Description

technical field [0001] The invention relates to the technical field of cell fermentation, in particular to a method for circulating fermentation of propionibacterium. Background technique [0002] Propionic acid is obtained by fermentation with propionibacterium, which can be used in food, stone, medicine and other fields. However, propionibacterium needs to cultivate seeds before entering the fermentation stage during the fermentation process. According to the growth situation, the seed tank cultivation needs to be expanded in multiple stages, so that the concentration can meet the requirements for entering the fermentation tank. After the seed tank is grown and transplanted into the fermenter, the fermenter also needs to be cultivated for a period of time. After the bacteria undergo the adaptation period and the logarithmic growth period, the concentration of the bacteria can become stable, and a large amount of growth can produce the desired product. During the fermentat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/52C12R1/01
CPCC12P7/52
Inventor 刘仕英
Owner ZHUMADAIN TIANZHONG BIOTECH CO LTD
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