HPLC (High Performance Liquid Chromatography) method for simultaneously determining content of cucurbitacin and dihydrocucurbitacin F in hemsleya amabilis

A technology of gentamidin and gentamidin, which is applied in the field of HPLC, can solve problems such as single method, achieve the effect of improving quality control level and promoting development and utilization

Active Publication Date: 2019-02-01
四川辅正药业股份有限公司
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  • Abstract
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Problems solved by technology

[0003] At present, the quality research on snow gallbladder mainly uses snow gallbladder as the detection index, and the method is relatively single

Method used

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  • HPLC (High Performance Liquid Chromatography) method for simultaneously determining content of cucurbitacin and dihydrocucurbitacin F in hemsleya amabilis
  • HPLC (High Performance Liquid Chromatography) method for simultaneously determining content of cucurbitacin and dihydrocucurbitacin F in hemsleya amabilis
  • HPLC (High Performance Liquid Chromatography) method for simultaneously determining content of cucurbitacin and dihydrocucurbitacin F in hemsleya amabilis

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Embodiment 1

[0037] Embodiment 1 HPLC method of the present invention

[0038] Methods as below:

[0039] 1. Prepare the test solution:

[0040] Take 1g of snow gall powder (passed through No. 2 sieve), add 40ml of methanol precisely, ultrasonicate at 40°C for 50min, let it cool, make up for the weight loss, filter, take 2ml of the filtrate, centrifuge at 16000rpm for 10min, and take the supernatant to prepare the test solution .

[0041] 2. Prepare the reference solution

[0042] Accurately weigh 0.00232g of bildosin A, place it in a 5ml volumetric flask, add methanol to constant volume to 5ml, and make a 0.464mg / ml stock solution of eucalyptin; Place in a 5ml volumetric flask, add methanol to constant volume to 5ml, and make a stock solution of bilbilin with a concentration of 0.638mg / ml;

[0043] Precisely draw 1000ul of bilbacetin stock solution and 400ul of bilbacholin stock solution into the same 2ml volumetric flask, add methanol to make the volume to 2ml, and prepare bilbacelin...

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Abstract

The invention provides a HPLC method for simultaneously determining content of cucurbitacin and dihydrocucurbitacin F in hemsleya amabilis. The method comprises the following steps of: a, preparing atest solution; b, preparing a reference solution; c, respectively injecting the test solution and the reference solution into a high performance liquid chromatography for detection; and d, calculatingthe content of the cucurbitacin and the dihydrocucurbitacin F in the hemsleya amabilis according to the detection result. The HPLC method can simultaneously detect the content of the su-hemsleya in the hemsleya amabilis, can be used for identifying different base source varieties of the hemsleya amabilis, and lays a foundation for improving the quality control level of the hemsleya amabilis and promoting development and utilization of medicinal materials.

Description

technical field [0001] The invention relates to an HPLC method for simultaneously determining the contents of gentamidin and gentamidin in snow gall bladders. Background technique [0002] The snow gall contained in Chuanbiao is the dry tuber root of Hemsleyadolichocarpa W.J.Chang, Hemsleyadolichocarpa W.J.Chang, Hemsleyadolichocarpa W.J.Chang, Hemsleyadolichocarpa W.J.Chang, Hemsleyadolichocarpa W.J.Chang, or Hemsleyagigantha W.J.Chang. Excavated in late autumn and early spring, removed impurities, sliced ​​while fresh, and dried. Also known as the golden tortoise lotus, the bottom of the pot, it is commonly called the golden tortoise lotus in Sichuan Province. It can clear away heat and detoxify, reduce inflammation and relieve pain, and is used for bacillary dysentery, enteritis, bronchitis, acute tonsillitis, toothache due to deficiency fire, and stomach pain. This product has been recorded in successive dynasties of herbal medicines. "Materia Medica Recipe" is called...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/884
Inventor 孙继林姚仁川
Owner 四川辅正药业股份有限公司
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