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A kind of automatic bacteria picker and microorganism screening method

A technology for microbial screening and bacteria picking instrument, which is applied in the fields of biochemical equipment and methods, separation of microorganisms, enzymology/microbiology devices, etc. Ease of culturing and screening effects

Active Publication Date: 2020-09-08
BIOGAS SCI RES INST MIN OF AGRI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the microplate reader or turbidity meter on the market is relatively large, and the read data cannot be directly used by the pipetting workstation

Method used

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  • A kind of automatic bacteria picker and microorganism screening method
  • A kind of automatic bacteria picker and microorganism screening method
  • A kind of automatic bacteria picker and microorganism screening method

Examples

Experimental program
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Embodiment 1

[0038] An automatic bacteria picker, comprising:

[0039] Liquid handling station 1, such as Figure 1-2 As shown, the liquid pipetting workstation 1 includes a liquid suction module 11 and a Y-axis tray 12, the liquid suction module 11 is installed in the upper housing 13 of the liquid pipetting workstation 1, and the liquid suction module 11 is driven by the X-axis driver 14 to realize X Axis movement, the Y-axis tray 12 is installed on the loading plate 15 at the bottom of the pipetting workstation 1, the Y-axis tray 12 is driven by the Y-axis driver 16 to realize the Y-axis movement, and the Y-axis tray 12 is provided with 9 plate positions 17, The plate area 17 in the lower left corner is a hollow structure, and a microporous plate is installed in the plate area 17 in the lower left corner. In this embodiment, there are 384 micropores on the microporous plate. The liquid suction module 11 includes a plurality of pipetting heads 111 , and the pipetting heads 111 are drive...

Embodiment 2

[0044] On the basis of Example 1, this embodiment provides a screening method for screening a single bacterial strain based on the automatic bacteria picker of Example 1. The original sample of this embodiment is a complex sample containing intestinal anaerobic bacteria. This embodiment The purpose is to obtain a single bacterial strain with obvious growth in the original sample, and the method of the present embodiment comprises the following steps:

[0045] Step S1, prepare the original sample, dilute the original sample with the pipetting workstation of the automatic bacteria picker, add the diluted original sample to the YCFA medium to obtain a culture medium sample, and use the pipette workstation of the automatic bacteria picker to dilute the culture medium sample Dispense into microwells of 384 microwell plate;

[0046] Step S2, culture the medium sample in the microwell plate in a constant temperature incubator outside the automatic picker for 5 days and then grow the ...

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Abstract

The invention discloses an automatic bacteria picker and a microbial screening method. The automatic bacteria picker includes a liquid transfer workstation and a turbidity meter, and the turbidimeter is installed on the liquid transfer workstation. The invention also discloses a microbial picker based on the automatic bacteria picker. A screening method, which is used for the acquisition of a single bacterial strain. The pipetting workstation of the present invention is combined with a turbidity meter. Compared with the previous liquid pipetting workstation combined with a microplate reader, the measurement environment has a lower impact on the growth of microorganisms, so that the present invention can be used in anaerobic environments and other conditions that minimize exposure time. Under certain circumstances, it can also quickly and accurately measure and determine the growth of microorganisms; in addition, the turbidimeter can directly read the turbidity in the well and then generate the picking sequence file of the pipetting workstation for the pipetting workstation to pick microorganisms. The present invention is based on the screening method for obtaining a single bacterial strain provided by the automatic bacterial picking instrument, and separates a single bacterial strain from a complex sample by using the automatic bacterial picking instrument for screening, and obtains a single bacterial strain with obvious growth.

Description

technical field [0001] The invention relates to an automatic bacteria picker and a microorganism screening method thereof, in particular to a bacteria picker for high-throughput cultivation and screening of microorganisms and a method for using the bacteria picker to screen microorganisms. Background technique [0002] The liquid pipetting workstation is an instrument that can automatically transfer and dilute high-throughput liquid reagents. It can automatically complete the work of transfer, dilution and distribution according to the microplate type and the setting of meaningful well positions. This equipment is of great value in the high-throughput cultivation and screening of microorganisms. By using a microplate reader and other instruments to read the value of each well of the microplate, and then determine the growth of the microorganisms according to the read results and the threshold , and then manually set the holes that need to be extracted and transferred, which...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12M1/36C12M1/34C12N1/02
CPCC12M23/02C12M41/36C12N1/02
Inventor 承磊王权周正蔡颖鹏曾碗秋王琼张雪
Owner BIOGAS SCI RES INST MIN OF AGRI
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