Method for detecting and analyzing protopanoxadiol

A technology of protopanaxadiol and analysis method, which is applied in the direction of analysis materials, measuring devices, material separation, etc., can solve the problems of limitation of toxic and side effects, achieve high accuracy, improve detection efficiency and detection accuracy

Inactive Publication Date: 2019-02-15
HOBOOMLIFE BIO TECH SHENZHEN CO LTD
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

Many anticancer drugs, such as topoisomerase inhibitors, alkylating agents, antimetabolites and hormone antagonists, can induce apoptosis, but their toxic and side effects limit their clinical application

Method used

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  • Method for detecting and analyzing protopanoxadiol
  • Method for detecting and analyzing protopanoxadiol

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Embodiment Construction

[0020] In order to make those skilled in the art more clearly understand the purpose, technical solutions and advantages of the invention, the invention will be further elaborated below in conjunction with the accompanying drawings and embodiments.

[0021] A protopanaxadiol detection and analysis method, which uses high performance liquid chromatography for detection and analysis, respectively detects the sample to be tested and the reference substance of known concentration, and calculates according to the external standard method according to the detection results and the concentration of the reference substance. The content of protopanaxadiol in the sample to be tested; in the high performance liquid chromatography, the mobile phase used is water and acetonitrile, and gradient elution is performed within 0-45min; in the gradient elution process, the water in the mobile phase The percentage by volume decreased from 67% to 10% and then increased to 67%, and the percentage by ...

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Abstract

The invention relates to the technical field of medicine, and provides a method for detecting and analyzing protopanoxadiol. The method for detecting and analyzing protopanoxadiol comprises the following steps of: detecting and analyzing by using a high performance liquid chromatography; separately detecting a tested sample and a reference substance of a known concentration; calculating accordingto a detection result and the concentration of the reference substance by using an external standard method to obtain the content of protopanoxadiol in the tested sample. In the high performance liquid chromatography, the used mobile phase is water, acetonitrile, and a gradient elution is performed in 0-45 min. During the gradient elution, the volume percentage of water in the mobile phase is reduced from 67% to 10% and then increased to 67%, and the volume percentage of acetonitrile in the mobile phase is increased from 33% to 90% and then reduced to 33%. The detection method can accurately detect the content of ginsenoside PPD in the sample.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a detection and analysis method for protopanaxadiol. Background technique [0002] Ginseng is considered to be a famous and precious traditional Chinese medicine for thousands of years. Ginseng has the effects of warming and nourishing, and is considered a famous nourishing medicine. But, in fact, the efficacy of ginseng lies not only in nourishing, but also in its medicinal properties. The "Famous Doctors" edited by Tao Hongjing believes that ginseng also has a good therapeutic effect on various diseases caused by gastrointestinal function decline. Modern studies have found that ginseng also has anti-tumor effects, which has attracted more and more attention at home and abroad. [0003] Tumor has become a topic of widespread concern in recent years. Tumors range from one to many. Although the treatment methods are constantly improving, it is still a difficult problem that can...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/28
CPCG01N30/02G01N30/28
Inventor 陈建生刘喜元张慧梁倩李峰胡欢
Owner HOBOOMLIFE BIO TECH SHENZHEN CO LTD
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