Germination method for seeds containing endophytic fungi
An endophytic fungus and seed technology, applied in the fields of seed and rhizome treatment, application, agriculture, etc., can solve the problems of affecting plant growth and low plant bacteria content, and achieve the effect of increasing the bacteria content and seed germination rate
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Embodiment 1
[0016] Embodiment 1: Contain the germination of endophytic fungus Fescue chrysanthemum seed:
[0017] A kind of seed germination method containing endophytic fungus of the present embodiment is characterized in that: comprises the following steps:
[0018] (1) Put 10 grains of fescue seeds containing endophytic fungus into a kraft paper bag, and put the kraft paper bag into a dark environment at 3°C for 24 hours;
[0019] (2) Take out the seeds and place them at room temperature for 1 day;
[0020] (3) Pour the seeds into glass tubes, place 5 seeds per tube, and place them in a water bath environment at 45°C for 10 hours;
[0021] (4) Put the seeds into the hole tray according to 1 seed per hole, and carry out the germination of the seeds of the small glume fescue.
[0022] After testing, the germination rate of the fescue seeds containing the endophytic fungus treated by the method is 90%, and the germination rate of the seedlings is 100%.
Embodiment 2
[0023] Embodiment 2: Contain the germination of endophytic fungus Goose view grass seed:
[0024] A kind of seed germination method containing endophytic fungus of the present embodiment is characterized in that: comprises the following steps:
[0025] (1) Put 30 grains of E. argentina seeds containing endophytic fungi into a kraft paper bag, and put the kraft paper bag into a dark environment at 6°C for 48 hours;
[0026] (2) Take out the seeds and place them at room temperature for 3 days;
[0027] (3) Pour the seeds into glass tubes, place 15 seeds per tube, and place them in a water bath environment at 55°C for 20 hours;
[0028] (4) Put the seeds into the plug tray according to 2 seeds per hole, and carry out the germination of the seeds of Goose View Grass.
[0029] After testing, the germination rate of the seeds of the endophytic fungus-containing swan grass treated by the method is 91%, and the fungus-containing rate of the seedlings is 99.5%.
Embodiment 3
[0030] Embodiment 3: Contain the germination of endophyte ryegrass seed:
[0031] A kind of seed germination method containing endophytic fungus of the present embodiment is characterized in that: comprises the following steps:
[0032] (1) Put ryegrass seeds containing endophytic fungi into kraft paper bags according to 20 grains per bag, and put the kraft paper bags into a dark environment at 5°C for 36 hours;
[0033] (2) Take out the seeds and place them at room temperature for 2 days;
[0034] (3) Pour the seeds into glass tubes, place 10 seeds per tube, and place them in a water bath environment at 50°C for 15 hours;
[0035] (4) The seeds are put into the hole tray according to 1 seed per hole, and the ryegrass seeds are germinated.
[0036] After testing, the germination rate of the ryegrass seeds containing the endophytic fungus treated by the method is 92%, and the fungus-containing rate of the seedlings is 99.8%.
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