A SNP molecular marker related to growth traits of large white pigs and its application

A technology for growth traits and large white pigs, applied in the determination/inspection of microorganisms, recombinant DNA technology, biochemical equipment and methods, etc., to achieve the effects of large economic application value and scientific research value, speed up genetic progress, and save production costs

Active Publication Date: 2021-08-03
FOSHAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time, the research on DHRS4 gene mainly focuses on mice and human tumor cells, and there are few reports on pig muscle development, and the related molecular pathways and mechanisms in the pathways of pig growth traits still need further research

Method used

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  • A SNP molecular marker related to growth traits of large white pigs and its application
  • A SNP molecular marker related to growth traits of large white pigs and its application
  • A SNP molecular marker related to growth traits of large white pigs and its application

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Experimental program
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Effect test

Embodiment

[0024] 1. Experimental sample collection

[0025] 247 large white pigs from a certain pig farm in Shandong were randomly selected, and ear tissue samples were collected one by one, placed in 75% alcohol and stored at -20°C, to be extracted DNA.

[0026] 2. Extraction and detection of pig genomic DNA

[0027] (1) The pig ear tissue of sampled pig is cut, each 30 mg, shredded and placed in a 1.5 ml centrifuge tube respectively;

[0028] (2) Take a 50ml centrifuge tube, mix the proteinase K with a final concentration of 0.4mg / ml and the lysate evenly, and add 0.5ml of the lysate to the 1.5ml centrifuge tube containing the pig ear tissue for lysis;

[0029] (3) Place the centrifuge tube evenly on a constant temperature shaker (tightly seal the cap to avoid liquid leakage), place it overnight at 55°C, and digest until no tissue samples can be observed with the naked eye;

[0030] (4) After the sample is fully lysed, take out the centrifuge tube, add 0.3ml of saturated sodium chlo...

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Abstract

The invention discloses a SNP molecular marker related to growth traits of large white pigs and its application, belonging to the technical field of molecular marker breeding. The SNP molecular marker is located at the rs701332503 site of DHRS4 gene of Large White pig. The nucleotide sequence of the DHRS4 gene in the Large White pig is related to the growth traits of the Large White pig from the 5' end rs701332503 site, that is, the SNP molecular marker on the DHRS4 gene rs701332503 site of the Large White pig is related to the growth traits (feed conversion rate) of the Large White pig Correlation is a new molecular marker. By determining the genotype of the SNP locus of Large White pigs to be tested, the growth traits (feed conversion rate) of Large White pigs can be screened for breeding pigs, which can effectively save production costs and speed up genetic progress. Serving the screening of large white pigs, it can not only meet the huge market demand, but also has great economic application value and scientific research value.

Description

technical field [0001] The invention belongs to the technical field of molecular marker breeding, in particular to a SNP molecular marker related to growth traits of large white pigs and its application. Background technique [0002] Single Nucleotide Polymorphisms (Single Nucleotide Polymorphisms, SNP) is a base variation produced by a single nucleotide on the genome, which has the characteristics of large number of distributions and rich polymorphisms. Another important molecular genetic marker is called the third generation molecular genetic marker. The marker has been widely used in animal and plant marker-assisted breeding, gene mapping, genetic diversity research and other fields. [0003] Animal husbandry occupies a very important position in the development of modern agriculture, and its ratio in agricultural production is usually used as an important indicator to measure the development level of a country and region. Pork is the most important source of meat in Ch...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 杨言昭陈文坚郭照良朱晓萍
Owner FOSHAN UNIVERSITY
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