A SNP molecular marker related to growth traits of large white pigs and its application
A technology for growth traits and large white pigs, applied in the determination/inspection of microorganisms, recombinant DNA technology, biochemical equipment and methods, etc., to achieve the effects of large economic application value and scientific research value, speed up genetic progress, and save production costs
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[0024] 1. Experimental sample collection
[0025] 247 large white pigs from a certain pig farm in Shandong were randomly selected, and ear tissue samples were collected one by one, placed in 75% alcohol and stored at -20°C, to be extracted DNA.
[0026] 2. Extraction and detection of pig genomic DNA
[0027] (1) The pig ear tissue of sampled pig is cut, each 30 mg, shredded and placed in a 1.5 ml centrifuge tube respectively;
[0028] (2) Take a 50ml centrifuge tube, mix the proteinase K with a final concentration of 0.4mg / ml and the lysate evenly, and add 0.5ml of the lysate to the 1.5ml centrifuge tube containing the pig ear tissue for lysis;
[0029] (3) Place the centrifuge tube evenly on a constant temperature shaker (tightly seal the cap to avoid liquid leakage), place it overnight at 55°C, and digest until no tissue samples can be observed with the naked eye;
[0030] (4) After the sample is fully lysed, take out the centrifuge tube, add 0.3ml of saturated sodium chlo...
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