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Transient capillary tube isokinetic electrophoresis device and transient capillary tube isokinetic electrophoresis method based on voltage polarity conversion

A voltage polarity, isotachophoresis technology, applied in measurement devices, material analysis by electromagnetic means, instruments, etc., can solve the problem of transient capillary isotachophoresis separation window and peak capacity reduction, separation and concentration The effect is reduced, Extend the sample concentration time and other problems to achieve the effect of improving the detection and separation effect, increasing the length of the separation window, and improving the detection sensitivity

Pending Publication Date: 2019-03-08
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, a further increase in the injection volume of transient capillary isotachophoresis will result in a decrease in separation and concentration
Transient capillary isotachophoresis is to concentrate and then separate on the same capillary. Increasing the sample volume will shorten the length of the remaining capillary for concentration and separation; increasing the sample volume will also prolong the sample concentration time. On the one hand, the sample It cannot be completely concentrated. On the other hand, during the concentration process, the sample zone moves towards the outlet direction, which further shortens the length of the capillary used for separation.
Therefore, further increasing the injection volume will result in the reduction of the separation window and peak capacity of transient capillary isotachophoresis, the decrease of resolution, and the deterioration of the concentration effect.
Thus, the injection volume limit for transient capillary isotachophoresis is limited to around 30% of the column volume

Method used

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  • Transient capillary tube isokinetic electrophoresis device and transient capillary tube isokinetic electrophoresis method based on voltage polarity conversion
  • Transient capillary tube isokinetic electrophoresis device and transient capillary tube isokinetic electrophoresis method based on voltage polarity conversion
  • Transient capillary tube isokinetic electrophoresis device and transient capillary tube isokinetic electrophoresis method based on voltage polarity conversion

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Effect test

Embodiment 1

[0032] A transient capillary isotachophoresis device based on voltage polarity conversion, comprising: a polarity-reversible high-voltage power supply 1 , a gas sampling device 2 , a capillary 3 , a first sample bottle 4 and a second sample bottle 5 . The first sample bottle 4 is provided with a metal electrode 6 with one end inserted into the holding solution, and the other end of the metal electrode 6 is electrically connected to the high-voltage power supply 1 with reversible polarity, so as to realize the loading and supply of high voltage for electrophoresis. DC high voltage with reversible polarity. The sampling end of the capillary 3 is inserted into the containing liquid of the first sample bottle 4, the outlet end is inserted into the containing liquid of the second sample bottle 5, and the two ends of the capillary 3 are connected with the external detector 7, and the second sample The liquid contained in the bottle 5 is grounded through a metal wire 8 for the curren...

Embodiment 2

[0034] like figure 1 As shown, a transient capillary isotachophoresis device based on voltage polarity conversion, the rest of the structure is the same as the first embodiment, the difference is: in this embodiment, the detector 7 is an ultraviolet-visible light detector, and the capillary 3 The outlet end of the tube is inserted into the second sample bottle 5 filled with the background electrolyte after passing through the ultraviolet-visible light detector. In other embodiments, other detectors can be selected, and the structure can be adaptively changed.

[0035]In this embodiment, a digital barometer 10 for monitoring the air pressure of the sample injection is arranged between the gas sampling device 2 and the air pressure sampling sealing module 9 , so that the liquid flow rate and volume of the sample injection can be controlled.

[0036] In this embodiment, the gas sampling device 2 communicates with the digital barometer 10 and the air pressure sampling sealing modu...

Embodiment 3

[0040] A kind of transient capillary isotachophoresis method based on voltage polarity conversion of the present invention, comprises:

[0041] Through the method of polarity reversal, the sample ions move back and forth in the capillary in the way of capillary isotachophoresis and concentrate.

[0042] In this embodiment, the following steps are specifically included:

[0043] ①Put the background electrolyte in the first sample bottle, connect the device, start the gas sampling device, load the background electrolyte in the first sample bottle into the capillary by gas pressure injection, and make the background electrolyte filled with capillaries;

[0044] ② Replace the first sample bottle containing the sample solution, load the sample solution into the capillary by gas pressure injection, and control the injection volume of the sample solution by controlling the injection time of the gas sampling device;

[0045] ③ Replace the first sample bottle with the background elec...

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Abstract

The invention discloses a transient capillary tube isokinetic electrophoresis device and a transient capillary tube isokinetic electrophoresis method based on voltage polarity conversion. The transient capillary tube isokinetic electrophoresis device is characterized in that the device comprises a high-voltage power supply with reverse polarity, a gas sampling device, a capillary tube, a first sample bottle and a second sample bottle; a metal electrode with one end inserted into stored liquid is arranged in the first sample bottle, and the other end of the metal electrode is electrically connected with the high-voltage power supply with the reversible polarity; the inlet end of the capillary tube is inserted into the stored liquid of the first sample bottle, the outlet end of the capillarytube is inserted into stored liquid of the second sample bottle, and the portion between the two ends of the capillary tube is connected to an external detector; the stored liquid of the second sample bottle is grounded through a wire, the gas sampling device and the first sample bottle are communicated for air pressure sampling, and an air pressure sampling sealing module is arranged between thegas sampling device and the first sample bottle. The transient capillary tube isokinetic electrophoresis device has the advantages that through the voltage polarity conversion, the sampling volume can be increased, the concentration effect is good, separation windows are long, and the separation and detection effect of transient capillary tube isokinetic electrophoresis is improved.

Description

technical field [0001] The invention relates to the field of capillary electrophoresis, in particular to a transient capillary isotachophoresis device and method based on voltage polarity conversion. Background technique [0002] Capillary electrophoresis has the advantages of high separation efficiency, fast separation speed, and the ability to separate isomers. It has been widely used in many fields such as proteomics, metabolomics, drug analysis, and food testing. [0003] Injection volume is one of the main reasons that limit the further widespread application of capillary electrophoresis compared to liquid chromatography. The injection volume of traditional capillary zone electrophoresis is only about 1% of the volume of the capillary column. According to the calculation of a typical capillary with an inner diameter of 30 microns and a length of 1 meter, the injection volume of the sample is only 7 nanoliters. Pre-concentration of samples is an effective method to incr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447
CPCG01N27/447
Inventor 吴焕铭唐科奇
Owner NINGBO UNIV
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