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A kind of selenium-enriched Bifidobacterium longum and its preparation method and application

A Bifidobacterium longum and selenium-enriched technology, applied in the biological field, can solve problems such as poor kidney function and poor improvement effect, and achieve the effects of lowering serum insulin levels, lowering blood sugar levels, and reducing pathological lesions.

Active Publication Date: 2022-07-15
JIANGSU TARGET BIOMEDICINE RES INST +1
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the shortcomings in the prior art that the improvement effect of diabetes is not good and it is easy to cause poor kidney function, the purpose of the present invention is to provide a selenium-enriched Bifidobacterium longum and its preparation method and to delay the progress of diabetes in the preparation applications in medicine or food

Method used

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  • A kind of selenium-enriched Bifidobacterium longum and its preparation method and application
  • A kind of selenium-enriched Bifidobacterium longum and its preparation method and application
  • A kind of selenium-enriched Bifidobacterium longum and its preparation method and application

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Embodiment 1

[0035] (1) Preparation of bacterial liquid

[0036] Bifidobacterium longum was cultured anaerobically at 37°C TPY. Sodium selenite was dissolved in 200ml TPY medium to a final concentration of 25g / mL (the concentrations of sodium selenite were tried at 20, 22.5, 25, 27.5, 30g / mL, and the effects were similar); Bifidobacterium longum was Bacterial liquid, according to the amount of bacterial liquid taken: selenium-containing medium=1:25 (tried the mass ratio of Bifidobacterium longum bacterial liquid and selenium-containing medium to be 1:20, 1:22.5, 1:25, 1 : 27.5, 1:30, the effect is similar) inoculated into the new TPY medium; under anaerobic conditions, cultivate overnight in a constant temperature incubator at 37 ° C until OD600 reaches 0.2 and above, and collect the cultured bacterial liquid; under 3500rpm rotating speed, Centrifuge at 4°C for 5 min, and wash three times with 5% glucose saline. The collected bacterial solution was resuspended in 0.1 mL of 13% skim milk ...

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Abstract

The invention belongs to the field of biotechnology, and specifically discloses a selenium-enriched Bifidobacterium longum and a preparation method and application thereof. The selenium-enriched Bifidobacterium longum prepared according to the preparation method of the present invention can be applied to the preparation of medicines or foods for delaying the progression of diabetes, specifically including: preparing and improving the physiological indicators and metabolic conditions of diabetes, improving the insulin signaling pathway of the diabetes model, and improving the diabetic liver , pathological changes of pancreas and kidney, improve diabetic serum creatinine and blood urea nitrogen levels, prevent diabetic renal complications or renal function damage in the application of drugs or foods.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a selenium-enriched Bifidobacterium longum, a preparation method thereof, and an application in the preparation of medicines or foods for delaying the progression of diabetes. Background technique [0002] Bifidobacterium is a genus of gram-positive, non-motile, branched-chain anaerobes that grow naturally in the human gastrointestinal tract and protect the host against viral infection. Typically, bifidobacteria are used as probiotics to aid digestion. Today's scientific research has discovered and proven new uses for probiotics in metabolic disorders including diabetes. [0003] Selenium is a basic trace element required for human and animal health, and it is mainly involved in animal physiological processes in the form of selenoproteins. Selenium has been reported to continuously improve glucose homeostasis, thereby regulating glucose metabolic processes such as glycolysis and gl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/38A61K35/745A61P3/10A23L33/135A23L33/16C12R1/01
CPCA61P3/10A23L33/135A23L33/16C12N1/20C12N1/38A23V2002/00A61K2035/115A23V2200/30A23V2200/328A23V2250/1626
Inventor 华子春林艳张燕徐根兴
Owner JIANGSU TARGET BIOMEDICINE RES INST
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