Application of rhizoma alismatis extract in preparation of drug for relaxing airway smooth muscle

Abstract

The invention discloses the application of a rhizoma alismatis extract in the preparation of a drug for relaxing the airway smooth muscle. It is found that the rhizoma alismatis extract has an efficient function of relaxing the airway smooth muscle through the detection of the isolated tracheal ring muscle tension of an experimental mouse, can be used for alleviating the symptom of airway obstruction during asthmatic attack of a patient, and has great promotion value.

Description

technical field

[0001] The invention belongs to the technical field of medicine, and in particular relates to the application of Alisma alisma extract in the preparation of medicine for relaxing airway smooth muscle. Background technique

[0002] Asthma is caused by a variety of cells including airway inflammatory cells and structural cells (such as eosinophils, mast cells, T lymphocytes, neutrophils, smooth muscle cells, airway epithelial cells, etc.) and cellular components (cellular elements) involved in airway chronic inflammatory diseases. This chronic inflammation leads to airway hyperresponsiveness, usually with widespread and variable reversible airflow limitation, and causes recurrent symptoms of wheezing, shortness of breath, chest tightness, or coughing, often occurring and exacerbated at night and / or in the morning , Most patients can relieve spontaneously or after treatment. The risk factors of asthma include host factors (genetic factors) and environmental fa...

Examples

Embodiment 1

[0019] The preparation of embodiment 1 Alisma extract

[0020] Such as figure 1 As shown, take the dried tuber of Alisma medicinal material, crush it, add 85% ethanol according to the ratio of 1:10 and soak at room temperature for 1W, reflux extraction to obtain the extract, and the extract is evaporated to dryness under reduced pressure to obtain the crude extract of Alisma (namely Alisma ethanol extract), the extract of the crude extract of Alisma is extracted with petroleum ether, and the extracted organic phase is evaporated to dryness under reduced pressure and concentrated into an extract, which is the Alisma petroleum ether extract. The aqueous phase separated during the petroleum ether extraction is further added with ethyl acetate for extraction, and the extracted organic phase is evaporated to dryness under reduced pressure and concentrated into an extract, which is the ethyl acetate extract of Alisma alisma. The water phase separated during ethyl acetate extraction...

Embodiment 2

[0022] Example 2 Alisma extract relaxes the pre-contraction of airway smooth muscle induced by high potassium

[0023] 1. In vitro isolation of mouse trachea:

[0024] Male adult BALB / c mice of similar age were sacrificed by cervical dislocation, and the mice were fixed on the dissecting board with the abdomen facing up, and the chest cavity was cut open to expose the heart and lung tissue. After the heart was removed, a section from the Adam's apple to the lung For the whole tissue, immediately soak the removed tissue in PSS solution (NaCl 135mM, KCl 5mM, MgCl 2 1mM, CaCl 2 2mM, HEPES 10mM, glucose 10mM, NaOH to adjust the pH to 7.4), and then transferred to the gel plate to further separate the tracheal ring. Under a stereomicroscope, use Venus scissors to carefully remove the connective tissue around the trachea and the residual blood in the lumen, and separate the main tracheal ring of about 5-7mm.

[0025] 2. The tension detection method of mouse airway smooth muscle...

Embodiment 3

[0028] Example 3 Alisma extract relaxes the pre-contraction of airway smooth muscle induced by acetylcholine

[0029] Except that the high potassium solution (H-K) was replaced with 80 mM high potassium solution (H-K) or 100 μM acetylcholine (ACh) solution for pre-stimulation after equilibration, the rest of the steps were the same as in Example 2.

[0030] Such as image 3 , The isolated mouse tracheal rings experienced stable and continuous pre-contraction in 100 μMACh solution, and the tension of the contracted tracheal rings decreased gradually after adding Alisma extract in a concentration-dependent manner until it dropped to the baseline level before contraction. It shows that Alisma extract can basically completely relax acetylcholine and cause the contraction of airway smooth muscle, and the drug effect is concentration-dependent.

technical field

[0001] The invention belongs to the technical field of medicine, and in particular relates to the application of Alisma alisma extract in the preparation of medicine for relaxing airway smooth muscle. Background technique

[0002] Asthma is caused by a variety of cells including airway inflammatory cells and structural cells (such as eosinophils, mast cells, T lymphocytes, neutrophils, smooth muscle cells, airway epithelial cells, etc.) and cellular components (cellular elements) involved in airway chronic inflammatory diseases. This chronic inflammation leads to airway hyperresponsiveness, usually with widespread and variable reversible airflow limitation, and causes recurrent symptoms of wheezing, shortness of breath, chest tightness, or coughing, often occurring and exacerbated at night and / or in the morning , Most patients can relieve spontaneously or after treatment. The risk factors of asthma include host factors (genetic factors) and environmental fa...

Examples

Embodiment 1

[0019] The preparation of embodiment 1 Alisma extract

[0020] Such as figure 1 As shown, take the dried tuber of Alisma medicinal material, crush it, add 85% ethanol according to the ratio of 1:10 and soak at room temperature for 1W, reflux extraction to obtain the extract, and the extract is evaporated to dryness under reduced pressure to obtain the crude extract of Alisma (namely Alisma ethanol extract), the extract of the crude extract of Alisma is extracted with petroleum ether, and the extracted organic phase is evaporated to dryness under reduced pressure and concentrated into an extract, which is the Alisma petroleum ether extract. The aqueous phase separated during the petroleum ether extraction is further added with ethyl acetate for extraction, and the extracted organic phase is evaporated to dryness under reduced pressure and concentrated into an extract, which is the ethyl acetate extract of Alisma alisma. The water phase separated during ethyl acetate extraction...

Embodiment 2

[0022] Example 2 Alisma extract relaxes the pre-contraction of airway smooth muscle induced by high potassium

[0023] 1. In vitro isolation of mouse trachea:

[0024] Male adult BALB / c mice of similar age were sacrificed by cervical dislocation, and the mice were fixed on the dissecting board with the abdomen facing up, and the chest cavity was cut open to expose the heart and lung tissue. After the heart was removed, a section from the Adam's apple to the lung For the whole tissue, immediately soak the removed tissue in PSS solution (NaCl 135mM, KCl 5mM, MgCl 2 1mM, CaCl 2 2mM, HEPES 10mM, glucose 10mM, NaOH to adjust the pH to 7.4), and then transferred to the gel plate to further separate the tracheal ring. Under a stereomicroscope, use Venus scissors to carefully remove the connective tissue around the trachea and the residual blood in the lumen, and separate the main tracheal ring of about 5-7mm.

[0025] 2. The tension detection method of mouse airway smooth muscle...

Embodiment 3

[0028] Example 3 Alisma extract relaxes the pre-contraction of airway smooth muscle induced by acetylcholine

[0029] Except that the high potassium solution (H-K) was replaced with 80 mM high potassium solution (H-K) or 100 μM acetylcholine (ACh) solution for pre-stimulation after equilibration, the rest of the steps were the same as in Example 2.

[0030] Such as image 3 , The isolated mouse tracheal rings experienced stable and continuous pre-contraction in 100 μMACh solution, and the tension of the contracted tracheal rings decreased gradually after adding Alisma extract in a concentration-dependent manner until it dropped to the baseline level before contraction. It shows that Alisma extract can basically completely relax acetylcholine and cause the contraction of airway smooth muscle, and the drug effect is concentration-dependent.

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