Elimination of Asynchronous Fading Fluorescence in Raman Spectroscopy

Abstract

A method for eliminating asynchronously faded fluorescence in Raman spectroscopy, relating to a method for eliminating fluorescence in Raman spectroscopy, the method is to perform continuous multiple spectral measurements on samples, form multidimensional arrays of multiple collected spectra, and use ICA The algorithm calculates; input the number of ICA source signals, increase sequentially from 2, and output the separated signals; then calculate the information entropy of each output signal, when the minimum entropy value of all outputs appears, this entropy value is the global minimum entropy ; The separation signal corresponding to the global minimum entropy value is used as a Raman spectrum signal reduction output, that is, the fluorescence-eliminated Raman spectrum signal. The invention can solve the problems of asynchronous fading of fluorescence in Raman spectrum, judgment of source signal quantity in ICA, and randomness of ICA output results, and is easy to popularize and use.

Description

technical field [0001] The invention relates to a method for eliminating fluorescence in Raman spectrum, in particular to a method for eliminating asynchronous fading fluorescence in Raman spectrum. Background technique [0002] Fluorescence is an important reason for interfering with the measurement of Raman spectroscopy. In the prior art closest to this application, one is to use the fading effect of fluorescence weakening with the irradiation time to realize the elimination of synchronous fading fluorescence. It is assumed that in the system There is a single source of fluorescence, and within a small period of time, the fluorescence intensity decreases synchronously with time. When the fluorescence fading microelement is found, the fluorescence contained in the system can be found by inverse difference, and then subtracted, so as to obtain the fluorescence without interference. Raman signal. Another existing technology is to use the information entropy of the discrete s...

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a method for eliminating asynchronous fading fluorescence in Raman spectroscopy, so as to solve the problems of asynchronous fading of fluorescence in Raman spectroscopy, the problem of judging the number of source signals in ICA, ICA output randomness problem

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