Method for eliminating asynchronous faded fluorescence in Raman spectrums

Abstract

The invention discloses a method for eliminating asynchronous faded fluorescence in Raman spectrums, and relates to a method for eliminating fluorescence in Raman spectrums. According to the method, continuous repeated spectral measurement is carried out on a sample, a multi-dimensional array is formed by repeatedly acquired spectrums, and calculation is carried out by adoption of an ICA algorithm; an ICA source signal quantity is input, increase is carried out in sequence from 2, and separated signals are output; an information entropy of each output signal is solved, and when a minimum entropy value appears, the entropy value is a global minimum entropy; and a separated signal corresponding to the global minimum entropy value is restored and output as a Raman spectral signal, namely, a fluorescence eliminated Raman spectral signal. The method is capable of solving the problem of asynchronous fluorescence fading, ICA source signal number judgement and ICA output result randomness in Raman spectrums, and is easy to popularize and apply.

Description

technical field [0001] The invention relates to a method for eliminating fluorescence in Raman spectrum, in particular to a method for eliminating asynchronous fading fluorescence in Raman spectrum. Background technique [0002] Fluorescence is an important reason for interfering with the measurement of Raman spectroscopy. In the prior art closest to this application, one is to use the fading effect of fluorescence weakening with the irradiation time to realize the elimination of synchronous fading fluorescence. It is assumed that in the system There is a single source of fluorescence, and within a small period of time, the fluorescence intensity decreases synchronously with time. When the fluorescence fading microelement is found, the fluorescence contained in the system can be found by inverse difference, and then subtracted, so as to obtain the fluorescence without interference. Raman signal. Another existing technology is to use the information entropy of the discrete s...

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Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a method for eliminating asynchronous fading fluorescence in Raman spectroscopy, so as to solve the problems of asynchronous fading of fluorescence in Raman spectroscopy, the problem of judging the number of source signals in ICA, ICA output randomness problem

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