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Application of oncostatin-M as biomarker for preparing sepsis diagnosis reagent

A technology of oncostatin and sepsis, which is applied in the field of application of oncostatin M as a biomarker in the preparation of sepsis diagnostic reagents, and can solve the problems of lack of early diagnostic markers for sepsis

Inactive Publication Date: 2019-06-14
CHONGQING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the shortcomings of the prior art described above, the purpose of the present invention is to provide the application of Oncostatin M as a biomarker in the preparation or screening of sepsis diagnostic reagents, to solve the lack of sepsis in the prior art. Issues such as markers for early diagnosis

Method used

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  • Application of oncostatin-M as biomarker for preparing sepsis diagnosis reagent
  • Application of oncostatin-M as biomarker for preparing sepsis diagnosis reagent
  • Application of oncostatin-M as biomarker for preparing sepsis diagnosis reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Detection of OSM Protein Concentration in Serum of Patients with Sepsis

[0039]Serum samples from healthy subjects and patients with sepsis were collected (see Table 1), and ELISA kits (purchased from Abcam, product name Human Oncostatin M ELISA Kit, product number ab215543) were used to detect the expression of Oncostatin M, and the operation method Strictly follow the instructions attached to the kit. SPSS 19.0 software was used for statistical analysis, and GraphPadPrism 5.0 software was used for graphing.

[0040] Table 1 Statistical results of adult patients with sepsis and healthy subjects

[0041]

[0042]

[0043] Figure 1a Shown is the results of detection concentration of OSM protein in the serum of patients with clinical sepsis, Figure 1b Shown are the results of detection concentration of OSM protein in the serum of sepsis survivors and sepsis dead patients. The results showed that compared with 32 healthy donors, the concentration of OSM in the ...

Embodiment 2

[0045] Correlation analysis between OSM and PCT and WBC

[0046] According to the detected value of OSM protein in Example 1, correlation analysis was performed with the corresponding PCT and WBC values ​​collected clinically. SPSS 19.0 software was used for statistical analysis, and GraphPad Prism 5.0 software was used for graphing.

[0047] Figure 2a It is shown as a correlation analysis chart between OSM and conventional inflammatory factors PCT, Figure 2b It is shown as the correlation analysis chart of OSM and conventional inflammatory factors WBC. The results showed that the concentration of OSM in the serum of patients with sepsis was significantly positively correlated with PCT value and WBC value. , indicating that OSM has value as a reference index of inflammation in the diagnosis of sepsis.

Embodiment 3

[0049] ROC curve comparative analysis of serum OSM, PCT and WBC for the diagnosis of sepsis

[0050] According to the septic patients OSM, PCT and OSM values ​​collected in Examples 1 and 2, use SPSS19.0 software to draw the ROC curve, and use MedCalc15.8 software to compare the statistical difference of the area under the ROC curve, the analysis results are shown in Table 2 shown.

[0051] image 3 It is shown as the ROC curve comparative analysis of OSM, PCT and WBC in sepsis diagnostic effect analysis chart. The results showed that: the area under the curve (AUC) of OSM concentration in serum was 0.94, the cutoff value was 10.52pg / ml, the sensitivity was 85.71%, and the specificity was 100%, better than WBC (P<0.05), When OSM and PCT, and OSM, PCT and WBC are used for combined diagnosis, the diagnostic benefit is better than that of PCT (P = 0.025, 0.023) and WBC (P = 0.0015, 0.0018), respectively. Therefore, we believe that OSM+PCT Combined, OSM+WBC combined, OSM+PCT+WB...

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Abstract

The invention provides an application of oncostatin-M as biomarker for preparing sepsis diagnosis reagent. The invention provides a condition that the oncostatin-M can be used as a diagnosis index ofthe sepsis; the oncostatin-M can be used as an index for assessing the severity degree of the sepsis, and the treatment and / or prognosis of the sepsis can be predicted according to the oncostatin-M expression level.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of oncostatin M as a biomarker in the preparation of sepsis diagnostic reagents. Background technique [0002] The latest guidelines for sepsis in 2017 define it as a life-threatening multiple organ dysfunction caused by the body's anti-infection immune dysregulation. Sepsis is a global public health emergency and one of the highest causes of death in the world. About 31.5 million people suffer from sepsis every year, and more than 5.3 million of them eventually die. [0003] The pathogenesis of sepsis is very complex. Scholars such as M.J.Delano believe that when the host initially fails to control the infection or overdos it, the cascade of inflammatory factors is released in a cascade, and a large amount of pro-inflammatory factors are released simultaneously with the synthesis and release of anti-inflammatory factors. , is closely related to the subsequent devel...

Claims

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Application Information

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IPC IPC(8): G01N33/68
Inventor 曹炬颜星星
Owner CHONGQING MEDICAL UNIVERSITY
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