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Stable culture method for simulating algal blooms indoors

A cultivation method and technology of algae bloom, applied in the field of algae cultivation, can solve the problems of differences in physiological properties of microalgae in natural water bodies, difficulty in capturing the physiological state of algae cells, and large errors in research results of microalgae, achieving good application prospects and short cultivation cycles , low cost effect

Active Publication Date: 2019-06-18
CHONGQING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of the above-mentioned deficiencies existing in the prior art, the object of the present invention is to provide a stable cultivation method for indoor simulated algal blooms, which solves the problem that the existing indoor cultivation method has a single type of microalgae and differences in physiological properties and microalgae in natural water bodies. Problems that lead to large errors in microalgae research results and difficulty in capturing the physiological state of algae cells

Method used

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  • Stable culture method for simulating algal blooms indoors
  • Stable culture method for simulating algal blooms indoors
  • Stable culture method for simulating algal blooms indoors

Examples

Experimental program
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Effect test

Embodiment 1

[0035] A stable culture method for indoor simulated water blooms, comprising the following steps:

[0036] 1) Collect 1000 mL of water at a depth of 0.5 m from the surface of the Microcystis bloom lake, remove impurities visible to the naked eye, and seal it for later use.

[0037] 2) After collecting natural water and filtering it through a 0.45μm membrane, measure the concentration of TN and TP in it, and then add 0.5mg / mLNaNO to it 3 (as N), 0.05mg / mlKH 2 PO 4 (calculated as P) and pure water, adjust TN to 0.5mg / L, TP to 0.05mg / L, finally high-temperature sterilization, and obtain microalgae culture solution after cooling;

[0038] Inoculate the microalgae obtained in step 1) into 2L of the microalgae culture solution in a sterile environment, and place it in a light incubator for 4 days. 12h: 12h, ventilation 100 ml / min; then the expanded microalgae were centrifuged at 5000rpm for 5min, the supernatant was discarded, and the microalgae culture solution was used to rinse...

Embodiment 2

[0056] A stable culture method for indoor simulated water blooms, comprising the following steps:

[0057] 1) Collect 1000 mL of water at a depth of 0.5 m from the surface of the Microcystis bloom lake, remove impurities visible to the naked eye, and seal it for later use.

[0058] 2) After collecting natural water and filtering it through a 0.45μm membrane, measure the concentration of TN and TP in it, and then add 0.5mg / mLNaNO to it 3 (as N), 0.05mg / mlKH 2 PO 4 (in terms of P) and pure water, adjust TN to 0.5mg / L, TP to 0.05mg / L, and finally high-temperature sterilization, after cooling, the microalgae culture solution is obtained, and then the obtained in step 1) The microalgae were inoculated into 2L of the microalgae culture solution, and placed in a light incubator for 4 days. The culture conditions were 25°C, 2000-2500 lux light, 12h:12h light-to-dark ratio, and 100 ml / min ventilation. Then the expanded microalgae were centrifuged at 5000 rpm for 5 min to discard the...

Embodiment 3

[0062] A stable culture method for indoor simulated water blooms, comprising the following steps:

[0063] 1) Collect 1000 mL of water at a depth of 0.5 m from the surface of the Microcystis bloom lake, remove impurities visible to the naked eye, and seal it for later use.

[0064] 2) After collecting natural water and filtering it through a 0.45μm membrane, measure the concentration of TN and TP in it, and then add 0.5mg / mLNaNO to it 3 (as N), 0.05mg / mlKH 2 PO 4 (in terms of P) and pure water, adjust TN to 0.5mg / L, TP to 0.05mg / L, and finally high-temperature sterilization, after cooling, the microalgae culture solution is obtained, and then the obtained in step 1) The microalgae were inoculated into 2L of the microalgae culture solution, and placed in a light incubator for 4 days. The culture conditions were 25°C, 2000-2500 lux light, 12h:12h light-to-dark ratio, and 100 ml / min ventilation. Then the expanded microalgae were centrifuged at 5000 rpm for 5 min to discard the...

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Abstract

The invention discloses a stable culture method for simulating algal blooms indoors. An outdoor natural water body is taken as inlet raw water, outdoor algal bloom microalgae which are not separated are taken as an algae source, the cost of nutritive salt is low, the culture cycle is short, the obtained microalgae have the cell concentration in the range of 6.68*10<4>-512.17*10<4> cells / mL and theChl a reaching 11.24-197.39 mu g / L, and the cell concentration and Chl a reach the definition standard of the algal blooms; the obtained microalgae are mixed microalgae and have high photosynthetic activity, outdoor algal blooms are simulated to a certain extent, a novel method for stably culturing mixed microalgae indoors is realized, and a stable microalgae source is provided for research of the follow-up algal blood forming mechanism and control measures. Further, in combination with a chemostat culture device, indoor continuous and stable culture of the microalgae is realized. The methodcan be used for stable culture of the mixed microalgae, can be used for researching the competition relation between different microalgae and has good application prospects.

Description

technical field [0001] The invention relates to the technical field of algae cultivation methods, in particular to an indoor stable cultivation method for simulating algal blooms. Background technique [0002] Algal blooms refer to a natural ecological phenomenon in which algae multiply in fresh water bodies. rich water body Nutritization It is a characteristic, mainly due to the waste water containing a large amount of nitrogen and phosphorus in domestic and industrial and agricultural production entering the water body, blue algae (aka Cyanobacteria ,include Microcystis , Anabaena , Oscillator algae , Nostoc , Cyanococcus, Nostoc Wait), Chlorella , diatoms A phenomenon in which the water body appears blue or green after a large number of reproductions. Algae bloom is an environmental problem faced by many countries around the world. In many nutrient-rich lakes or reservoirs, algae multiply abnormally, accumulate in local lake areas or reservoir areas,...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12M1/00C12M1/04C12M1/02C12R1/89
Inventor 郭劲松石小娟张树青方芳晏鹏李奕璇
Owner CHONGQING UNIV
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