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A kind of stable cultivation method for indoor simulated algae bloom

A cultivation method and water bloom technology, applied in the field of algae cultivation, can solve the problems of difficulty in capturing the physiological state of algae cells, differences in physiological performance of microalgae in natural water bodies, large errors in research results of microalgae, etc., achieving good application prospects and short cultivation period , low cost effect

Active Publication Date: 2021-07-20
CHONGQING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of the above-mentioned deficiencies existing in the prior art, the object of the present invention is to provide a stable cultivation method for indoor simulated algal blooms, which solves the problem that the existing indoor cultivation method has a single type of microalgae and differences in physiological properties and microalgae in natural water bodies. Problems that lead to large errors in microalgae research results and difficulty in capturing the physiological state of algae cells

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  • A kind of stable cultivation method for indoor simulated algae bloom
  • A kind of stable cultivation method for indoor simulated algae bloom
  • A kind of stable cultivation method for indoor simulated algae bloom

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0035] A stable culture method for indoor simulated water blooms, comprising the following steps:

[0036] 1) Collect 1000 mL of water at a depth of 0.5 m from the surface of the Microcystis bloom lake, remove impurities visible to the naked eye, and seal it for later use.

[0037] 2) After collecting natural water and filtering it through a 0.45μm membrane, measure the concentration of TN and TP in it, and then add 0.5mg / mLNaNO to it 3 (as N), 0.05mg / mlKH 2 PO 4 (calculated as P) and pure water, adjust TN to 0.5mg / L, TP to 0.05mg / L, finally high-temperature sterilization, and obtain microalgae culture solution after cooling;

[0038] Inoculate the microalgae obtained in step 1) into 2L of the microalgae culture solution in a sterile environment, and place it in a light incubator for 4 days. 12h: 12h, ventilation 100 ml / min; then the expanded microalgae were centrifuged at 5000rpm for 5min, the supernatant was discarded, and the microalgae culture solution was used to rinse...

Embodiment 2

[0056] A stable culture method for indoor simulated water blooms, comprising the following steps:

[0057] 1) Collect 1000 mL of water at a depth of 0.5 m from the surface of the Microcystis bloom lake, remove impurities visible to the naked eye, and seal it for later use.

[0058] 2) After collecting natural water and filtering it through a 0.45μm membrane, measure the concentration of TN and TP in it, and then add 0.5mg / mLNaNO to it 3 (as N), 0.05mg / mlKH 2 PO 4 (in terms of P) and pure water, adjust TN to 0.5mg / L, TP to 0.05mg / L, and finally high-temperature sterilization, after cooling, the microalgae culture solution is obtained, and then the obtained in step 1) The microalgae were inoculated into 2L of the microalgae culture solution, and placed in a light incubator for 4 days. The culture conditions were 25°C, 2000-2500 lux light, 12h:12h light-to-dark ratio, and 100 ml / min ventilation. Then the expanded microalgae were centrifuged at 5000 rpm for 5 min to discard the...

Embodiment 3

[0062] A stable culture method for indoor simulated water blooms, comprising the following steps:

[0063] 1) Collect 1000 mL of water at a depth of 0.5 m from the surface of the Microcystis bloom lake, remove impurities visible to the naked eye, and seal it for later use.

[0064] 2) After collecting natural water and filtering it through a 0.45μm membrane, measure the concentration of TN and TP in it, and then add 0.5mg / mLNaNO to it 3 (as N), 0.05mg / mlKH 2 PO 4 (in terms of P) and pure water, adjust TN to 0.5mg / L, TP to 0.05mg / L, and finally high-temperature sterilization, after cooling, the microalgae culture solution is obtained, and then the obtained in step 1) The microalgae were inoculated into 2L of the microalgae culture solution, and placed in a light incubator for 4 days. The culture conditions were 25°C, 2000-2500 lux light, 12h:12h light-to-dark ratio, and 100 ml / min ventilation. Then the expanded microalgae were centrifuged at 5000 rpm for 5 min to discard the...

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Abstract

The invention discloses an indoor stable cultivation method for simulating algal blooms. The natural water body in the field is used as the influent raw water, and the unseparated algal microalgae in the field are used as the algae source. The cell concentration at 6.68×10 4 ~512.17×10 4 In the range of cells / mL, Chl a reached 11.24~197.39μg / L, all of which reached the definition standard of water bloom, and the obtained microalgae were mixed microalgae with high photosynthetic activity, which simulated the field water bloom to a certain extent, and realized the A new method for stably cultivating mixed microalgae indoors provides a stable source of algae for subsequent research on the formation mechanism and control measures of water blooms. Furthermore, combined with the chemostat culture device, the continuous and stable culture of indoor microalgae is realized. This method can not only be used for the stable cultivation of mixed microalgae, but also be used for the study of the competition relationship among different microalgae, which has a good application prospect.

Description

technical field [0001] The invention relates to the technical field of algae cultivation methods, in particular to an indoor stable cultivation method for simulating algal blooms. Background technique [0002] Algal blooms refer to a natural ecological phenomenon in which algae multiply in fresh water bodies. rich water body Nutritization It is a characteristic, mainly due to the waste water containing a large amount of nitrogen and phosphorus in domestic and industrial and agricultural production entering the water body, blue algae (aka Cyanobacteria ,include Microcystis , Anabaena , Oscillator algae , Nostoc , Cyanococcus, Nostoc Wait), Chlorella , diatoms A phenomenon in which the water body appears blue or green after a large number of reproductions. Algae bloom is an environmental problem faced by many countries around the world. In many nutrient-rich lakes or reservoirs, algae multiply abnormally, accumulate in local lake areas or reservoir areas,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12M1/00C12M1/04C12M1/02C12R1/89
Inventor 郭劲松石小娟张树青方芳晏鹏李奕璇
Owner CHONGQING UNIV
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