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Potato residue culture medium

A medium and potato technology, applied in the field of microorganisms, can solve the problems of incompetent price, high cost, low yield, etc., and achieve the effects of shortening culture time, low cost and high yield

Active Publication Date: 2014-06-11
BEIJING GUANLAN TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in recycling, potato waste often faces the following problems: 1) Potato waste is highly viscous and difficult to separate solid and liquid; 2) Sterilization of potato waste before recycling is time-consuming and expensive
As we all know, in the field of microbial engineering, from small-scale experimental screening to industrial production above the pilot scale, the conditions of each link must be optimized, including the optimization of the culture medium. The experimental conditions are mechanically expanded to large-scale production, and it will be impossible to implement due to the controllability of the pH value, temperature and gas required for cultivation.
In addition, the technical obstacles of bacterial cellulose mainly lie in the low level of fermentation, low yield, high cost, and the price is not equal to ordinary plant cellulose; the second is that further research and utilization of bacterial cellulose film-forming and molding technology has not yet been resolved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Preparation of potato waste residue

[0018] In the present invention, the potato waste residue is the residue after extracting starch from fresh potato products. The specific preparation method is: taking potatoes, cleaning the soil on the surface of the potatoes, and removing foreign objects such as rocks and iron filings. Use a pulverizer to crush the washed potatoes into a paste until they are twisted by hand without graininess. A proper amount of clean water is injected to accelerate the flow of potato dregs and make starch and potato dregs separate better. The potato meal is sieved with a vibration frequency of 130 times / min, and the sieve openings are 30 mesh, 50 mesh, 60 mesh, and 80 mesh from the front to the back. The starch flows into the sieve, and the potato waste flows out from the sieve. The humidity is 75%. Other methods can also be used to separate starch in potatoes to obtain potato waste residue.

[0019] To remove potato residues, heat for ...

Embodiment 2

[0020] Example 2 Preparation of bacterial cellulose from potato waste residue culture solution

[0021] 1 Preparation of bacterial cellulose

[0022] Acetobacter xylinum strains were inoculated into the culture medium for recovery culture, dynamic shaker culture, 30℃, 2-3 days; the BC fermentation broth after dynamic enrichment was transferred to a shallow dish, 30℃, static culture 14 Heaven, get BC, it is film-like. Place the BC obtained from the culture in distilled water at 90°C for 2 hours, wash it with distilled water twice after taking it out; boil it with a boiling 0.5 mol / L NaOH solution for 15 minutes; put it in a 1% NaOH solution statically Leave it for 2 days; soak it in deionized water until it is neutral to obtain BC, which is a BC wet film; freeze-dry the wet film and store it to obtain BC, which is a BC dry film.

[0023] 2 Potato culture solution

[0024] Take potato waste residue and add water to warm it at 100°C (80°C is also possible) for 1 hour to obtain potato ...

Embodiment 3

[0030] Example 3 Glycerin culture medium of potato waste residue

[0031] In Example 2, a single potato waste residue is used as the culture medium, and the yield of bacterial cellulose is 3.0-3.3%; the potato waste residue is the residue left after most of the starch in the potato is extracted, and the bacterial cellulose Growth requires an appropriate amount of carbon and nitrogen sources. Therefore, if an appropriate amount of carbon source can be added to the potato residue, the yield of bacterial cellulose can be increased. When selecting carbon sources, three conditions must be met: one is the low price, otherwise the low-cost advantage of waste recycling will be lost; the other is that it must be able to adapt to industrialized large-scale production through laboratory scale enlargement Cultivation can still maintain the stability of the flora; thirdly, the yield is high. If the yield of the combined use of potato waste residue and other carbon sources (based on productio...

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PUM

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Abstract

The invention relates to a bacterial cellulose culture medium, which is a potato culture fluid. The technical scheme of the potato culture fluid provided by the invention specifically comprises the steps of: taking potato residues, adding water, and heating at a temperature of 80-100 DEG C for not less than 1 hour to make the mass ratio of potato to distilled water 2-11:50, so as to obtain the potato culture fluid, wherein the pH value of the potato culture fluid is 4.0-7.5; and mixing glycerin and the potato culture fluid to obtain a culture fluid, wherein the mass ratio of glycerin to potato in the potato culture fluid is 2-13:20-110. According to the invention, the potato residues after potato starch production are firstly used as a raw material for a culture medium and are treated by using the simplest method to obtain the culture medium for culturing bacterial cellulose; the culture medium has low cost and high yield rate, the problem of recycling the potato residues is simultaneously solved, and the culture time is short; in addition, the invention provides an important condition for stable and large-scale bacterial cellulose culture.

Description

technical field [0001] The invention relates to the field of microbes, in particular to a culture medium prepared by reusing waste. Background technique [0002] Potatoes are widely distributed, highly adaptable, and easy-to-cultivate crops with high yields. In addition to being used as food, potatoes are mainly used for starch processing. Every ton of starch produced from potatoes will produce 7.5 tons of potato waste. Potato waste residue mainly contains protein, amino acid and a small amount of sugar, which is a recyclable resource. At present, potato waste residue can be recycled for the production of alcohol, feed, degradable plastics, extraction of pectin and preparation of dietary fiber. However, in recycling, potato waste often faces the following problems: 1) Potato waste is highly viscous and difficult to separate solid and liquid; 2) Sterilization of potato waste before recycling is time-consuming and expensive. [0003] Bacterial cellulose is another kind of c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/02C12R1/02
Inventor 吴力克
Owner BEIJING GUANLAN TECH
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