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Neural stem cell line

A technology of neural stem cells and cells, applied in the field of neural stem cells and culture conditions for culturing neural stem cells, to achieve the effects of saving working time, enhancing activity, and simple preparation methods

Inactive Publication Date: 2017-12-15
温州市鹿城区中津先进科技研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The culture methods of neural stem cells known in the art (due to the above reasons) are not suitable for these research and therapeutic applications

Method used

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Embodiment Construction

[0018] Below by specific embodiment the present invention is described in further detail.

[0019] The methods used in the embodiments of the present invention include nerve tissue preservation solution, nerve tissue washing solution, nerve tissue decomposition solution, cell washing solution, primary cell culture solution, cell digestion solution, subculture cell culture solution, cell cryopreservation solution, etc. Wherein, the secondary cell culture medium is a serum-containing growth medium for neural stem cells, which consists of 1000ml basal medium, 50ug / ml fetal bovine serum, 10ng / ml bFGF, 10ng / ml EGF, 0.5mmol / ml ml glutamine, 0.5mmol / ml sodium pyruvate mixed composition. The secondary cell culture medium is the obtained neural stem cell adherence culture medium, which enables the neural stem cells to adhere to the wall and have stronger activity. The nerve tissue preservation solution is a culture medium containing one or more of RPMI-1640, DMEM, a-MEM and DMEM / F12 c...

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Abstract

The present invention relates to neural stem cell lines maintained in an adherent monolayer culture in the presence of EGF and FGF-2, wherein no more than 1% of the cells express mature astrocytes, neurons or A marker for oligodendrocytes, which are not derived from human embryonic stem cells. The preparation method of the invention is simple and easy to operate.

Description

technical field [0001] The invention relates to neural stem cells and the culture conditions and methods for cultivating neural stem cells. In particular neural stem cell lines are provided. Background technique [0002] Although neural stem cells have been said to be isolated from a variety of sources in vitro, it has so far not been possible to expand cells in large-scale culture in a symmetrically dividing, undifferentiated state. Long-term expansion of cells in this state is highly desirable from both an experimental and a therapeutic point of view. The resulting pure neural stem cell population was capable of directed differentiation into three cell types: neurons, astrocytes, and oligodendrocytes. [0003] One known method of culturing neural stem cells (albeit as a small fraction of a heterogeneous cell population) is the neurosphere system. This system consists of serially passaged heterogeneous aggregates of cells, only a small fraction of which are true neural s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0797C12R1/91
CPCC12N5/0623
Inventor 王振宇
Owner 温州市鹿城区中津先进科技研究院
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