Anti-human NGF antibody and its preparation method and use

Abstract

The invention provides an antibody combined with NGF or an antigen-binding fragment thereof, and lists the amino acid sequences of the heavy chain and light chain variable regions of the antibody. The NGF antibody or antigen-binding fragment thereof provided by the invention has higher affinity to NGF and can effectively block the binding between NGF receptors and NGF. The antibody or its antigen-binding fragment can inhibit the binding activity of NGF and receptors in vitro, and is suitable for treating pain diseases related to the hyperexpression and level increase of NGF.

Description

technical field [0001] The present invention relates to the field of therapeutic monoclonal antibodies, more particularly, to an anti-NGF antibody or an antigen-binding fragment thereof and uses thereof. Background technique [0002] Nerve Growth Factor (NGF) is the first neurotrophic factor to be discovered. It has the dual biological functions of neuron nutrition and neurite outgrowth. The expression of characteristics has an important regulatory role. NGF contains three subunits, α, β, and γ. The β subunit is the active subunit of NGF that plays the role of nerve regeneration and repair. Currently known NGF receptors include a high-affinity tyrosine kinase receptor (Tropomyosin receptor kinase A, TrkA) and a low-affinity p75 neurotrophin receptor (p75Neurotrophin Receptor, p75NTR). [0003] Although initially the primary role of NGF was to promote neuronal survival and differentiation, an increasing number of studies over the last two decades have shown that NGF is asso...

AI Technical Summary

Problems solved by technology

The immunogenicity of different CDRs is different, resulting in different antibody tolerance speed and toxicity, which directly affects the efficacy of the drug

On the other hand, the subject's immune response to the antibody itself will form immune complexes, which will alter the pharmacokinetics and produce allergic reactions, etc., eliminating its therapeutic use

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