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Preparation method for human umbilical cord mesenchymal stem cell preparation used for conventional anti-aging treatment

A mesenchymal stem cell and anti-aging technology, which is applied in the field of preparation of human umbilical cord mesenchymal stem cell preparations, can solve the problems of technology to be perfected and difficult technical links, and achieve good cryopreservation effect, guaranteed survival rate and high expression rate Effect

Inactive Publication Date: 2019-07-02
和携科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still many technical difficulties that need to be overcome, especially the technology of stem cell storage needs to be perfected

Method used

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  • Preparation method for human umbilical cord mesenchymal stem cell preparation used for conventional anti-aging treatment
  • Preparation method for human umbilical cord mesenchymal stem cell preparation used for conventional anti-aging treatment

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Embodiment 1

[0020] A method for preparing a human-derived umbilical cord mesenchymal stem cell preparation for conventional anti-aging treatment, comprising the steps of obtaining umbilical cord tissue block, the step of primary culture of stem cells, the step of subculture of stem cells and the preparation of human umbilical cord mesenchymal stem cells preparation steps.

[0021] The steps of obtaining the umbilical cord tissue block include sterilizing the ligated umbilical cord at both ends, and placing it in physiological saline containing 1wt% double antibody; The umbilical cord tissue segment was rinsed repeatedly with normal saline containing 1wt% double antibody to remove blood stains; the umbilical cord tissue segment was cut open, blood vessels and epidermal tissue were removed to obtain Wharton's jelly tissue, and normal saline containing 1wt% double antibody was used to treat the umbilical cord tissue. Tong's jelly tissue was rinsed repeatedly, and cut into pieces of umbilical...

Embodiment 2

[0036] A preparation method for a human-derived umbilical cord mesenchymal stem cell preparation for conventional anti-aging treatment, comprising the steps of obtaining umbilical cord tissue blocks, the step of primary culture of stem cells, the step of subculture of stem cells, the step of cryopreservation of stem cells, the step of storing stem cells The steps of recovering and culturing, the step of subculture of stem cells and the step of preparing human umbilical cord mesenchymal stem cell preparation.

[0037] The steps of obtaining the umbilical cord tissue block include sterilizing the ligated umbilical cord at both ends, and placing it in physiological saline containing 1wt% double antibody; The umbilical cord tissue segment was repeatedly rinsed with normal saline containing 1wt% double antibody to remove blood; the umbilical cord tissue segment was cut open, blood vessels and epidermal tissue were removed to obtain Wharton's jelly tissue, and normal saline containin...

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Abstract

The invention relates to a preparation method for a human umbilical cord mesenchymal stem cell preparation used for conventional anti-aging treatment. The method includes the following steps: acquisition of an umbilical cord tissue block, primary culture of stem cells, subculture of the stem cells, cryopreservation of the stem cells, recovery and culture of the stem cells, and preparation of the human umbilical cord mesenchymal stem cell preparation; and the step of the preparation of the human umbilical cord mesenchymal stem cell preparation includes the following steps: resuspension subculture is carried out to Pe-generation human umbilical cord mesenchymal stem cell by employing 5% of glucose injection, after a Pe-generation human umbilical cord mesenchymal stem cell suspension is uniform, 5% of the glucose injection is supplemented, the concentration of the Pe-generation human umbilical cord mesenchymal stem cell is adjusted to 4*10<4>-6*10<4> / mL to obtain the human umbilical cordmesenchymal stem cell preparation; and wherein e=6, 7, 8, 9, and 10. The preparation method improves reservation of human umbilical cord mesenchymal stem cells, and ensures the survival rate, the proliferation capability and the specificity of the human umbilical cord mesenchymal stem cells.

Description

technical field [0001] The invention relates to the technical field of biomedicine, more specifically, it relates to a preparation method of a human umbilical cord mesenchymal stem cell preparation used for conventional anti-aging treatment. Background technique [0002] Aging is an inevitable development trend of organisms and a complex and changeable process. Throughout the life process, the body will be affected and affected by various substances, energies, and information in the external environment, and tissues and organs will inevitably suffer damage and functional decline. With the improvement of medical technology and living standards, the number of aging population is increasing. Along with aging, the physiological functions of human organs are affected, such as hypertension, coronary atherosclerosis and other diseases. Delaying aging may reduce the occurrence of these diseases, improve the functional status of the body as a whole, and thus improve the quality of l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775A01N1/02A61K35/28A61P39/06
CPCA01N1/021A61K35/28A61P39/06C12N5/0665C12N2509/00
Inventor 赵进军谷广其杨桂花赵宇飞李张鹏
Owner 和携科技有限公司
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