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Piglet intestinal mucosa protective agent and its preparation method and application

An intestinal mucosa and protective agent technology, applied in the field of piglet intestinal mucosa protective agent, can solve the problems affecting the quality of animal products and human health, environmental pollution, resource waste, etc., to improve the level of antioxidant protein, broad application prospects, and production materials. easy-to-get effect

Active Publication Date: 2020-11-17
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Zinc can prevent and treat diarrhea and improve growth performance in production. It is widely used as a feed additive. However, the extensive use of inorganic zinc has caused waste of resources and environmental pollution, affecting the quality of animal products and human health.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1, a kind of preparation method of piglet intestinal mucosa protective agent, comprises the following steps:

[0029] 1), using fresh pig manure to prepare fecal suspension:

[0030] Prepared according to Example 1 of 201710439896.7 "Fecal flora transplantation technology and its application"; specifically, the following steps are carried out in sequence:

[0031] 1. Feces collection and screening conditions: Collect fresh feces from Jinhua pigs, a local pig breed in my country, for use. The screening conditions for donor pigs are:

[0032] (1) Diarrhea has not occurred and antibiotics (any antibiotics) have not been used within 2-3 months until the date of collection.

[0033] (2) Collect Nikkei feces to detect the pathogens of swine fever, porcine circovirus type 2 infection, blue ear disease, pseudorabies, swine erysipelas, swine mycoplasma pneumonia, foot-and-mouth disease and other diseases.

[0034] 2. Preparation of fecal suspension

[0035] (1) fir...

Embodiment 2

[0050] Embodiment 2, a kind of preparation method of piglet intestinal mucosa protective agent,

[0051] Step 1), 2) are the same as in Example 1.

[0052] 3), preparation of piglet intestinal mucosa protective agent:

[0053] According to the ratio (volume ratio) of 0.5:99.5, the zinc-loaded chitosan suspension is slowly added under stirring (100r / min) to (the time of slowly adding is 1 to 2 minutes) in the prepared feces and bacteria suspension, Place it on ice for 3 minutes under continuous stirring (100r / min), adjust the pH of the reaction mixture to 7.4, and distribute the obtained piglet intestinal mucosa protective agent into sterile centrifuge tubes, and store at -80°C.

[0054] Experiment 2. The freshly prepared piglet intestinal mucosa protective agent obtained in Example 2 was tested according to the method described in Experiment 1;

[0055] The results showed that compared with the control group, the daily gain of piglets in the experimental group increased by 1...

Embodiment 3

[0058] Embodiment 3, a kind of preparation method of piglet intestinal mucosa protective agent,

[0059] Step 1), 2) are the same as in Example 1.

[0060] 3), preparation of piglet intestinal mucosa protective agent:

[0061] According to the ratio (volume ratio) of 1:99, the zinc-loaded chitosan suspension was slowly added under stirring (150r / min) to the feces suspension prepared (the time of slow addition was 1 to 2 minutes), Place it on ice for 5 minutes under continuous stirring (150r / min), adjust the pH of the reaction mixture to 7.2, and distribute the obtained piglet intestinal mucosa protective agent into sterile centrifuge tubes, and store at -80°C.

[0062] Experiment 3. The freshly prepared piglet intestinal mucosa protective agent obtained in Example 3 was tested according to the method described in Experiment 1;

[0063] The results showed that compared with the control group, the daily gain of the piglets in the experimental group increased by 10.4% (P<0.05),...

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PUM

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Abstract

The invention discloses a preparation method of a piglet intestinal mucosa protective agent. The method includes preparing a fecal bacteria suspension by using fresh pig feces, preparing a zinc-loadedchitosan suspension, slowly adding the zinc-loaded chitosan suspension into the fecal bacteria suspension under stirring, and placing the mixture on ice for reaction for 3-8 minutes under the condition of continuous stirring; and adjusting the pH value to 7.0-7.4 after the reaction is finished to obtain the piglet intestinal mucosa protective agent, wherein the volume ratio of the fecal bacteriasuspension to the zinc-loaded chitosan suspension is 98: 2-99.5: 0.5. The piglet intestinal mucosa protective agent can be used as oral liquid for piglet, can enhance protective autophagy of piglet intestinal mucosa, improve antioxidant protein level, reduce inflammatory reaction, improve intestinal mucosa function, and also have zinc supplement and diarrhea prevention effects.

Description

technical field [0001] The invention relates to a protective agent for piglet intestinal mucosa, a preparation method and application thereof. Background technique [0002] In modernized and large-scale pig production, the stocking density is too high and the growth rate is too fast. The pigs are often in a state of high stress, which leads to the imbalance of intestinal flora and a series of intestinal diseases. Therefore, in the current pig production in my country, it is very important to maintain the intestinal health of piglets. [0003] The intestinal mucosa is not only the main site for the digestion and absorption of nutrients, but also the largest protective barrier for the body, which can prevent the invasion of pathogens and antigenic substances in the intestinal tract and protect the health of animals. The surface of the intestinal mucosa hosts a diverse flora that plays a key protective role against infection by modulating the immune system and mediating coloni...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/722A61K33/30A61K35/741A61P1/00A61P1/12A23K50/30A23K50/60A23K10/18A23K20/163A23K20/20
CPCA23K10/18A23K20/163A23K20/30A23K50/30A23K50/60A61K31/722A61K33/30A61K35/741A61P1/00A61P1/12A61K2300/00
Inventor 韩新燕马馨张雨琛
Owner ZHEJIANG UNIV
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