Verticillium dahliae endophyte and application thereof
A technology of Verticillium dahliae and endophytic bacteria, applied in the direction of application, bacteria, biocide, etc., can solve the problems of economic loss, lower cotton fiber quality, unsatisfactory disease prevention effect, etc., to avoid environmental pollution and protect plants cell effect
Active Publication Date: 2019-07-09
NANYANG NORMAL UNIV
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Problems solved by technology
Verticillium wilt not only reduces the yield of cotton, but also reduces the fiber quality, seed yield and seed oil production of cotton, which in turn causes serious economic losses
[0003] At present, the control of Verticillium wilt of cotton at home and abroad mainly adopts traditional methods such as crop rotation, breeding of disease-resistant varieties, chemical control, etc., but the effect of disease prevention is not ideal. These methods have certain limitations and shortcomings. poor adaptability or unsatisfactory effect
Method used
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Embodiment 1
[0021] Step 1), screen out the V1 strain from the resistant cotton leaves in the cotton test field of Nanyang Normal University, which has a strong antagonistic effect on Verticillium dahliae, after determining its antagonistic activity through multiple plate confrontation experiments, it has been On October 26, 2018, it was preserved in the China Center for Type Culture Collection (CCTCC, preservation address: Wuhan University Campus, No. 229 Bayi Road, Wuchang District, Wuhan City, Hubei Province, preservation number: CCTCC M 2018715, and the strain was identified and determined It is Enterobacter xiangfangensisV1 strain.
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The invention provides verticillium dahliae endophyte which is an Enterobacter xiangfangensis V1 strain with the collection number of CCTCC M 2018715. The Enterobacter xiangfangensis V1 strain has thefunction of inhibiting verticillium dahliae. The full length of peroxidase genes in Enterobacter is 603bp, 200 amino acids are encoded, and an expression product after heterogeneous expression of thegenes has the function of inhibiting the verticillium dahliae. A biocontrol technology is utilized to provide a microbial resource for cotton verticillium wilt control. Besides, compared with chemical control, the biocontrol technology avoids the problems of environmental pollution and the like.
Description
technical field [0001] The invention belongs to the technical field of biological control of plant diseases, and in particular relates to endophytic bacteria of Verticillium dahliae and application thereof. Background technique [0002] Cotton is an important fiber plant, Verticillium dahliae infection caused by Verticillium dahliae is one of the main diseases of cotton, known as "cotton cancer". The pathogenic bacteria have strong viability, and the sclerotia can survive for more than 10 years without a host in the soil. The pathogenic bacteria population is rich in genetic diversity and easy to mutate. In addition, the host range of Verticillium dahliae is very wide, foreign reports can damage 660 species of plants in 38 families, including 184 species of crops and 153 species of weeds. my country has identified at least 80 species of host plants in 20 families. Field crops include sunflower, eggplant, pepper, tomato, tobacco, potato, melon, watermelon, cucumber, peanut, ...
Claims
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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/19
CPCA01N63/00C12R2001/01C12N1/205
Inventor 张林陶冶陈俊梅李文鹏黄冰纷牛秋红
Owner NANYANG NORMAL UNIV
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