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Establishment method of hplc fingerprint of the dichloromethane part of yao medicinal material ginger

A kind of technology of Dichloromethane and Fingerprint of Leonago

Active Publication Date: 2021-08-20
GUANGXI UNIV OF CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, there is no relevant report on the fingerprint of Mother Ginger in the prior art both at home and abroad

Method used

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  • Establishment method of hplc fingerprint of the dichloromethane part of yao medicinal material ginger
  • Establishment method of hplc fingerprint of the dichloromethane part of yao medicinal material ginger
  • Establishment method of hplc fingerprint of the dichloromethane part of yao medicinal material ginger

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Embodiment 1

[0061] The method for establishing the HPLC fingerprint of the dichloromethane part of the Yao medicinal material Ginger Leonum L. comprises the following steps:

[0062] (1) Preparation of the test solution: Accurately weigh 2.0000 g of Yimu Jiang medicinal material, accurately add 50 ml of ethanol solution with a volume fraction of 70%, weigh the weight, ultrasonicate for 60 min, let it cool and weigh to make up for the lost weight , filtered, the filtrate was precisely added 20 ml of 3mol / ml hydrochloric acid solution, refluxed in a water bath for 2 h, let it cool, adjusted to neutrality with a 40% sodium hydroxide solution, evaporated to dryness in a water bath, and the residue was heated with 30 ml Dissolve in water and transfer to a separatory funnel to extract with dichloromethane, extract twice, 30 ml each time, combine the dichloromethane part, evaporate to dryness, dilute the residue to a 10 ml brown volumetric flask with methanol, and add 0.22 µm organic filter memb...

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Abstract

The invention discloses a method for establishing an HPLC fingerprint of the dichloromethane part of the Yao medicinal material Leonurus japonica, comprising the following steps: (1) preparing a test solution, (2) preparing a reference solution with diosgenin as a standard product, 3) HPLC detection: record the HPLC spectrum within 95min. (4) Detect multiple test samples, use the software "Similarity Evaluation System of Chromatographic Fingerprints of Traditional Chinese Medicine", and match all chromatographic peaks by the method of multi-point calibration, and automatically generate the control fingerprint R as the control fingerprint. By establishing the fingerprint of Leonurus japonica, the invention can more comprehensively reflect the relative relationship of the components contained in Leonurus japonica, and can also effectively distinguish medicinal materials from different origins, so as to better evaluate and control the quality thereof. It is beneficial to promote the development and utilization of Guangxi Yaoyao Leimujiang.

Description

technical field [0001] The invention relates to the technical field of fingerprints of traditional Chinese medicinal materials, in particular to a method for establishing the HPLC fingerprint of the dichloromethane part of the Yao medicinal material Zingiber officinalis. Background technique [0002] Zingiber officinale Costus speciosus (Koen.) Smith The rhizome of the perennial root herb, is one of the commonly used Yao medicine. The Yao people often use it as a nursing medicine for puerpera, and it has a good recovery effect on the puerpera, so the Yao people call it "Yimu Jiang" and "Guanyin Jiang". Growing in damp places such as valleys below 1700 m above sea level, forests or roadsides at forest edges, shrubs and grasses by streams. It is mainly distributed in Guangxi, Guangdong, Yunnan, Fujian, Taiwan and other provinces, and it is also distributed in Southeast Asia and subtropical regions. Guangxi Yimu Jiang is mainly distributed in Laibin, Baise, Guilin, Pingxiang...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/86
CPCG01N30/8686G01N30/88G01N2030/8809
Inventor 王志萍黄杰连黎芳苏佳昇李晓霞蒋雅娴刘笑
Owner GUANGXI UNIV OF CHINESE MEDICINE