A kind of animal model construction method and application of mycoplasma bovis infection guinea pig

A technology of mycoplasma bovis and its construction method, which is applied in the field of animal model construction of guinea pigs infected with mycoplasma bovis, can solve the problems of lack of animal models, slow progress, lack, etc., and achieve the effects of high economic cost, obvious price advantage, and convenient feeding and management

Active Publication Date: 2021-04-06
DAIRY CATTLE RES CENT SHANDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mycoplasma bovis pneumonia caused by calves infected with Mycoplasma bovis has caused serious economic losses to the cattle industry in my country due to poor curative effect of conventional drugs and no effective vaccine for prevention.
[0004] At present, Mycoplasma bovis has become a research hotspot of domestic and foreign cattle scientists, but the progress is slow
At the same time, due to the lack of commercialized Mycoplasma bovis vaccines in my country, it is impossible to effectively control the Mycoplasma bovis epidemic in pastures.
The inventors found that an important reason for the above two situations is the lack of suitable animal models for basic research and development of biological products
At present, there are only reports about the rabbit animal model of M. bovis at home and abroad, but no related reports about the guinea pig animal model of M. bovis.

Method used

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  • A kind of animal model construction method and application of mycoplasma bovis infection guinea pig
  • A kind of animal model construction method and application of mycoplasma bovis infection guinea pig
  • A kind of animal model construction method and application of mycoplasma bovis infection guinea pig

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1 guinea pig infection experiment

[0036] Mycoplasma bovis suspension for challenge: Mycoplasma bovis (Mycoplasma bovis) 16M strain was inoculated in PPLO liquid medium (purchased from BD Company) containing 20% ​​horse serum at a ratio of 1:10, placed in a 37°C incubator, Cultivate for 3-5 days until the culture medium turns from red to yellow, centrifuge at 12 000 g for 10 min, and collect Mycoplasma bovis cells. Resuspend in PBS and dilute to tube 10 in a 10-fold gradient. Pipette 50 μL of the suspension from tube 6, tube 7, tube 8, tube 9 and tube 10, spread it evenly on the solid culture plate of Mycoplasma bovis, and place it at 37°C, 10% CO 2Colony counts were performed after 5 days of culture under conditioned conditions. Prepare M. bovis suspension at the desired concentration according to the colony count results.

[0037] Select 12 weaned healthy guinea pigs of the Hartley strain at the age of 21, and randomly divide them into two groups: the c...

Embodiment 2

[0040] Embodiment 2 vaccine protection experiment

[0041] The guinea pig model prepared in Example 1 was used to evaluate the protective effect of the Mycoplasma bovis inactivated vaccine.

[0042] The 16M strain of Mycoplasma bovis (Mycoplasma bovis) was inoculated into the culture medium for expanded cultivation, the culture was harvested, the culture was inactivated, and after inactivation, an adjuvant was added and mixed to obtain an inactivated vaccine. After sterility test and allergen test, it is used for vaccine protection test.

[0043] Twelve 21-day-old healthy weaned guinea pigs were randomly divided into two groups: immune-challenge group and control-challenge group. The obtained Mycoplasma bovis inactivated vaccine was used to immunize the guinea pigs in the immunization-challenge group, and a second immunization was carried out 21 days after the first immunization, and the immunization dose was 0.2 mL / guinea. Fourteen days after the second immunization, as des...

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Abstract

The invention provides a method for constructing an animal model of guinea pigs infected with Mycoplasma bovis and an application thereof, belonging to the technical field of animal model construction. The guinea pig animal model infected by Mycoplasma bovis prepared by the present invention has a sensitive period of up to 42 days to Mycoplasma bovis, and simultaneously effectively simulates the symptoms of pneumonia in calves infected by Mycoplasma bovis, so it can be used for the evaluation of the protective effect of Mycoplasma bovis vaccine, especially the long-term protective effect, It has good practical application value.

Description

technical field [0001] The invention belongs to the technical field of animal model construction, and in particular relates to a method for constructing an animal model of guinea pigs infected with Mycoplasma bovis and an application thereof. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Mycoplasma bovis (Mycoplasma bovis) can cause calf pneumonia, arthritis, keratoconjunctivitis and other diseases after infecting beef or dairy cattle. Since American Hale et al first isolated Mycoplasma bovis from the milk of cows suffering from mastitis in 1961, Mycoplasma bovis has spread rapidly to cattle in various countries and regions in the world, which has caused great damage to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/02A61D7/00
CPCA01K67/02A61D7/00
Inventor 张亮王基隆朱曼玲蒋仁新杨宏军解晓莉程凯慧张云飞刘来兴
Owner DAIRY CATTLE RES CENT SHANDONG ACADEMY OF AGRI SCI
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