Automatic liquid injection method and device for detecting monogenic genetic diseases
A genetic disease, single-gene technology, applied in the field of automatic liquid injection methods and devices for detecting single-gene genetic diseases, can solve problems such as reducing the accuracy of detecting single-gene genetic diseases, being unable to be distributed on a detection chip, and unable to distribute liquid samples evenly, etc. problem, to achieve the effect of easy interpretation, high accuracy and improved efficiency
Inactive Publication Date: 2021-12-14
冷泉港生物科技股份有限公司 +1
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[0013] However, the bottom of the microtube has an inverted cone shape, the detection chip extends to the innermost part of the microtube, and the tip of even the smallest micropipette (i.e., a 10 μl micropipette) cannot fully extend into the micropipette. The innermost part of the test tube, so the liquid sample can only be distributed on the surface of the detection chip near the opening end of the micro test tube, and cannot be distributed on the surface of the detection chip near the innermost end of the micro test tube, resulting in the liquid sample not being evenly distributed on the surface of the micro test tube. Detects the entire surface of the chip, reducing the accuracy of detecting monogenic diseases
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[0057] The following describes the implementation of the present invention in more detail in conjunction with the drawings and component symbols, so that those skilled in the art can implement it after studying this specification.
[0058] see figure 1 , figure 1 is a flow block diagram of the method of the present invention. The invention provides an automatic injection method for detecting monogenic genetic diseases, comprising the following steps:
[0059] Step S1: a detection chip 10 has a plurality of small holes 11 (see Figure 12 ), the detection chip 10 is set in a chamber 21 of a micro test tube 20 . A guide 30 is inserted into the chamber 21 of the microtube 20, and a first end 31 of the guide 30 is close to an end of the detection chip 10, such as figure 2 , image 3 and Figure 4 shown.
[0060] see figure 2 , image 3 and Figure 4 , figure 2 is a schematic diagram of step S1 of the method of the present invention, wherein eight consecutive rows of P...
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An automatic liquid injection method for detecting monogenic genetic diseases, the micro test tube is positioned on the frame body, the guide is positioned on the fixing device, the sample receiving part is positioned on the mobile device and leans against the guiding part, and the liquid sample is injected into the sample receiving part The moving device drives the sample holder to move to the innermost side of the detection chip, so that the liquid sample is evenly distributed on the surface of the detection chip and penetrates into and distinguishes one or tens of thousands of small holes in the detection chip through capillary phenomenon. In this way, the present invention not only realizes the automatic injection and partitioning of samples for single-gene genetic diseases (such as spinal muscular atrophy), but also has an equal amount of liquid samples in each small hole of the detection chip to achieve digital gene copy number interpretation results. Improve the efficiency, detection accuracy, stability and reproducibility of detecting monogenic genetic diseases.
Description
technical field [0001] The invention relates to an automatic liquid injection method and device, in particular to an automatic liquid injection method and device for detecting monogenic genetic diseases. Background technique [0002] There are many kinds of monogenic diseases, such as spinal muscular atrophy (SMA), Duchenne muscular dystrophy (DMD), multiple endocrine neoplasia, ichthyosis, hemophilia and Some patients with polycystic kidney disease (Polycystic Kidney Disease, PKD) and so on. The following will take various methods for detecting spinal muscular atrophy as an example to illustrate the problems of various methods for detecting monogenic diseases. [0003] The incidence of spinal muscular atrophy (SMA) ranks second among all chromosomal recessive genetic diseases, and its fatality rate ranks first. Clinically, spinal muscular atrophy can be divided into three types according to the time of onset: type 1 (also known as Werdnig-Hoffmann disease), the onset time...
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IPC IPC(8): G01N1/14B01L3/02
CPCG01N1/14B01L3/021G01N2001/149
Inventor戴国銧林台珮江建宽李彦贤陈俞伶
Owner冷泉港生物科技股份有限公司