44 results about "Genetic heredity" patented technology

The invention described herein provides high throughput methods and reagents for generating transgenic animals (e.g., mammals) through introducing materials into gametes or preimplantation stage (e.g., one-cell embryo or zygotes) via electroporation, leading to genetically inheritable modification to the genome of the animal.

The invention discloses a neural network construction system and method based on a variable-length genetic algorithm, wherein the construction system includes a sequentially connected training set generation module, a network initialization module, a network training module, a network update module, a network optimization module, Network selection module, iteration number update module and network generation module. The general idea of ​​the construction method of this scheme is to generate variable-length chromosomes in the initialization step, and randomly add BN components to specific genes of each chromosome. Through the training on the training set, the neural network structure representation corresponding to each chromosome is obtained through training, and the offspring chromosomes are selected. Subsequently, in the crossover step, the length of the crossed chromosomes is not fixed, and a growth and shrinking strategy is used to generate daughter chromosomes. Then use the traditional mutation and environment selection operations to complete the selection of offspring chromosomes, repeat the above, and decode the obtained best chromosomes into the corresponding neural network architecture.

The invention described herein provides high throughput methods and reagents for generating transgenic animals (e.g., non-human mammals) through introducing a CRISPR / Cas9 system comprising a Cas9 protein into gametes or preimplantation stage embryos (e.g., one-cell embryos or zygotes) via multiple cycles (e.g., 4- 10 cycles) of electroporation, leading to genetically inheritable modification to the genome of the animal.

The invention discloses a method for measuring pig gene polymorphism and relevance between pig gene polymorphism and sow reproduction traits. The method includes the following steps of firstly, collecting a pig ear tissue sample; secondly, extracting ear tissue DNA; thirdly, amplifying RBP4 and BMP7 gene target segments through a PCR; fourthly, conducting SSCP analysis; fifthly, conducting bidirectional sequencing on a PCR amplification product; sixthly, conducting population genetics characteristic analysis; seventhly, measuring reproductive performance; eighthly, analyzing the relevance between detected RBP4 and BMP7 gene heritable variation and sow reproduction trait indexes through a mathematical model. The method has the advantages of being high in detecting efficiency and reliable in result.

The invention relates to a pyramiding breeding method utilizing the Sino-US rice distant hybridization and anther culture technology. Hybridization is carried out between rice materials which are very different from each other in genetic backgrounds, complementation of protogene is fast achieved by utilizing the anther culture haploid breeding technology and the pyramiding breeding technology, and the maximization of the heterosis is achieved. The genetic distance between cross parents can be lengthened, and the heterosis is enhanced. The output, the resistance and other favorable characters can be greatly improved either the indica type rice is hybridized with the American rice or the japonica rice is hybridized with the American rice. Complementation between favorable characters at home and abroad can be gradually achieved through the backcross technology and the pyramiding breeding technology. The stability of breeding materials is accelerated through haploid breeding of anther culture. The advantage of high yield per plant can be obtained, and the yield of a whole group is facilitated.

The present invention relates to the field of animal virology, and provides a strain of avian encephalomyelitis virus and its application, specifically provides a strain of avian encephalomyelitis virus, named AEV JN, and its preservation number is: CGMCC No.16385, It also provides the isolation and identification process of the virus strain. Through the genetic evolution analysis of the VP1 gene, it is confirmed that it is in the same branch as the internationally recognized AEV virulent strain VR strain, and has a close relationship. It fills in the relevant gaps in my country. As a vaccine strain, it can well complement the existing 1143 strains of live vaccine commonly used in my country, and provide better protection for my country's poultry breeding industry.

The invention discloses a method for assisted detection of bovine growth and carcass traits by genetic markers of bovine SERPINA3 gene and its application. Using the whole genome DNA of yellow bovine to be tested as a template, PCR amplifies a partial fragment of the bovine SERPINA3 gene, and performs gene expression by PCR-RFLP technology. Genetic variation detection, the detection method of the present invention is simple, fast, low cost, accurate and reliable detection results, suitable for large-scale genotyping of cattle SERPINA3 gene genetic variation sites, and assisting molecular markers of cattle growth and carcass traits Selection provides new genetic markers, which can speed up the establishment of cattle populations with excellent genetic resources.

The invention belongs to the technical field of genetics, and in particular relates to a method for rapid extraction of genomic DNA and rapid genotype identification suitable for map-based cloning. The genotype rapid identification method suitable for map-based cloning described in the present invention uses crop seedlings as verification samples, verifies with a general DNA extraction method, and performs PCR amplification by designing reasonable primers, which can quickly complete the rapid extraction of genomic DNA The rapid identification of genotypes greatly reduces duplication of labor, saves cost and time, and speeds up work progress. This method can also be used for reference in other crops.

The invention discloses an InDel molecular marker closely linked with a rice heading stage gene and its application. The upstream primer sequence of the molecular marker IN2 is SEQ ID NO.2, and the downstream primer sequence is SEQ ID NO.3; the InDel molecular marker IN2 is used in rice Application in genetic analysis of heading stage genes and gene fine positioning; the upstream primer sequence of the molecular marker IN10 is SEQ ID NO.6, and the downstream primer sequence is SEQ ID NO.7; the InDel molecular marker IN10 is used in rice heading stage gene Applications in genetic analysis and fine mapping of genes.