A rapid genotype identification method suitable for map-based cloning

A technology of map-based cloning and identification methods, which is applied in the field of genetics and can solve problems such as time-consuming and duplication of genotype identification methods
CN110777196BActive Publication Date: 2021-08-10MAIZE RES INST SHANDONG ACAD OF AGRI SCI
2 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
MAIZE RES INST SHANDONG ACAD OF AGRI SCI
Publication Date
2021-08-10

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention belongs to the technical field of genetics, and in particular relates to a method for rapid extraction of genomic DNA and rapid genotype identification suitable for map-based cloning. The genotype rapid identification method suitable for map-based cloning described in the present invention uses crop seedlings as verification samples, verifies with a general DNA extraction method, and performs PCR amplification by designing reasonable primers, which can quickly complete the rapid extraction of genomic DNA The rapid identification of genotypes greatly reduces duplication of labor, saves cost and time, and speeds up work progress. This method can also be used for reference in other crops.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the technical field of genetics, and in particular relates to a method for rapid extraction of genomic DNA and rapid genotype identification suitable for map-based cloning. Background technique

[0002] The acquisition of the draft genome of maize in February 2008 made maize the second crop successfully sequenced after rice. In 2009, American scientists completed the genome sequencing of maize inbred line B73, and its coverage reached 95% (Schnable et al.2009). It is predicted that the size of the maize genome is about 2,500MB, and the number of genes is about 50,000-60,000. The sequencing team has already stored the draft information of the maize genome in the Internet gene sequencing public database maizeGDB, and is constantly updating the data.

[0003] The large-scale sequencing of the maize genome is conducive to the development of enough molecular markers by using the genome sequence information. At the same time, the ge...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More