Method for Determination of Pig Gene Polymorphism and Its Correlation with Reproductive Traits of Sow
A technology of gene polymorphism and determination method, applied in the field of determination of pig gene polymorphism and its correlation with reproductive traits of sows, can solve problems such as blanks
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[0077] A method for determining the correlation between pig gene polymorphisms and reproductive traits of sows, comprising the steps of:
[0078] (1) collecting ear tissue samples of adult fertile sows;
[0079] (2) DNA extraction from ear sample tissue;
[0080] (3) PCR amplification of retinol-binding protein, that is, RBP4, and bone morphogenetic protein, that is, the BMP7 gene target fragment;
[0081] (4) Single-strand conformational polymorphism of the target fragment, that is, SSCP analysis;
[0082] (5) Two-way sequencing of PCR amplification products;
[0083] (6) Analysis of population genetic characteristics;
[0084] (7) Determination of reproductive performance;
[0085] (8) The relationship between the detected genetic variation of RBP4 and BMP7 genes and the reproductive traits of sows was analyzed by mathematical model.
[0086] According to the single nucleotide polymorphism of the target gene, that is, single nucleotide polymorphism: SNP and the correlat...
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