Breeding method of high-rice-blast-resistance rice intermediate materials
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An intermediate material, rice blast technology, applied in the field of rice intermediate material breeding, can solve the problems of low yield, weak resistance to rice blast, and difficulty in achieving high resistance to rice blast, and achieve rich genetic background, high resistance to rice blast, etc. sex-prominent effect
Active Publication Date: 2012-11-21
ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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Many breeders have attempted to breed varieties with high blast resistance through various methods, but they have achieved little succe
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Embodiment 1
[0024] Embodiment 1: below in conjunction with accompanying drawing ( figure 1 , figure 2 , image 3 ) to further illustrate the breeding method of rice intermediate materials with high blast resistance carrying 5 blast resistance genes (Table 1):
[0025] Table 1 The source of the resistance gene of the present invention
[0027] 1. If figure 1 As shown, there is a linkage phenomenon between the genes on the same chromosome and the traits they control. The recombination type is obviously lower than the parent combination type. The closer the distance between genes, the stronger the linkage strength. In order to achieve the aggregation of rice blast resistance genes, the linkage must be broken first to complete the aggregation of resistance genes on the same chromosome. F obtained by crossi...
Embodiment 2
[0033] Example 2: It is basically the same as Example 1, the difference is that the repeated cycle step (3) is added, and the following is combined with the attached Figure 4 Further illustrate the example of repeating the cycle step (3), continuously realizing the polymerization of new rice blast resistance genes, and breeding intermediate rice materials with high blast resistance.
[0034] The source of the new rice blast resistance gene: Zhaiyeqing 8, the chromosome 8 contains the resistance gene Pi-11.
[0035] Taking KDW201 (Pi-13, Pi-25, Pigm, chr6; Pi-4, Pi-20, chr12) as the female parent and Zhaiyeqing No. 8 (Pi-11, chr8) as the male parent, the female parent and F 1 In the next generation, through anther culture and molecular marker-assisted selection, the intermediate material KDW301, which aggregated six rice blast resistance genes, was selected.
[0036] Field appraisal of KDW301: rice seedling blast grade 0, ear stem blast grade 0;
[0037] KDW301 inoculation ...
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Abstract
The invention discloses a breeding method of high-rice-blast-resistance rice intermediate materials, and particularly provides a method for breeding the high-rice-blast-resistance rice intermediate materials with the advantages that molecular markers are adopted for assisting the selection, and the anther culture is adopted for accelerating the stabilization. The method provided by the invention has the advantages that through the hybridization of rice-blast-resistance genes between different gene sites, the rice-blast-resistance genes are continuously polymerized, and the bred intermediate materials have very high resistance on the rice blast. The selected intermediate materials for the rice-blast-resistance gene polymerization have very prominent rice blast resistance, and can be widely used for improving the rice varieties with good integral properties but poor rice blast resistance. The interlocking between the rice-blast-resistance genes and rice-blast-sensitive genes on other gene sites is broken through anther, and the rice-blast-resistance genes are polymerized. The selected rice-blast-resistance material genes have very rich genetic backgrounds, filial generations prepared by using the selected rice-blast-resistance material genes as parents have very prominent resistance on the rice blast, and the new rice varieties with high rice blast resistance can be more easily obtained through breeding.
Description
technical field [0001] The invention relates to a method for breeding intermediate rice materials, and specifically describes a method for breeding rice intermediate materials with high blast resistance through molecular marker-assisted selection and accelerated and stable anther culture. Background technique [0002] At present, rice blast is one of the most serious diseases that harm japonica rice, and the grain loss caused by rice blast accounts for 11%-30% of the world's grain loss. fundamental strategy. Many breeders have attempted to breed varieties with high blast resistance through various methods, but they have achieved little success. The main reason is that the parental materials used for hybridization are not strong against Effects against rice blast. Contents of the invention [0003] The purpose of the present invention is to provide a method for breeding rice intermediate materials with high blast resistance, and create a batch of intermediate rice materia...
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