Breeding method of high-rice-blast-resistance rice intermediate materials
An intermediate material, rice blast technology, applied in the field of rice intermediate material breeding, can solve the problems of low yield, weak resistance to rice blast, and difficulty in achieving high resistance to rice blast, and achieve rich genetic background, high resistance to rice blast, etc. sex-prominent effect
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Embodiment 1
[0024] Embodiment 1: below in conjunction with accompanying drawing ( figure 1 , figure 2 , image 3 ) to further illustrate the breeding method of rice intermediate materials with high blast resistance carrying 5 blast resistance genes (Table 1):
[0025] Table 1 The source of the resistance gene of the present invention
[0026] donor resistance gene chromosome TETEP Pi-4 12 Devil's Valley Pi-13 6 IR24 Pi-20 12 Gumei 2 Pi-25 6 Gumei 4 Pigm 6
[0027] 1. If figure 1 As shown, there is a linkage phenomenon between the genes on the same chromosome and the traits they control. The recombination type is obviously lower than the parent combination type. The closer the distance between genes, the stronger the linkage strength. In order to achieve the aggregation of rice blast resistance genes, the linkage must be broken first to complete the aggregation of resistance genes on the same chromosome. F obtained by crossi...
Embodiment 2
[0033] Example 2: It is basically the same as Example 1, the difference is that the repeated cycle step (3) is added, and the following is combined with the attached Figure 4 Further illustrate the example of repeating the cycle step (3), continuously realizing the polymerization of new rice blast resistance genes, and breeding intermediate rice materials with high blast resistance.
[0034] The source of the new rice blast resistance gene: Zhaiyeqing 8, the chromosome 8 contains the resistance gene Pi-11.
[0035] Taking KDW201 (Pi-13, Pi-25, Pigm, chr6; Pi-4, Pi-20, chr12) as the female parent and Zhaiyeqing No. 8 (Pi-11, chr8) as the male parent, the female parent and F 1 In the next generation, through anther culture and molecular marker-assisted selection, the intermediate material KDW301, which aggregated six rice blast resistance genes, was selected.
[0036] Field appraisal of KDW301: rice seedling blast grade 0, ear stem blast grade 0;
[0037] KDW301 inoculation ...
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