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Dominant Molecular Marker of Rice Blast Resistance Gene pi67(t) and Its Application

A rice blast resistance gene and molecular marker technology, applied in the field of crop molecular genetics and breeding, can solve the problems of blast resistance phenotype dependence, low breeding efficiency, and difficult identification, and achieve good polymorphism, low cost, and high recombination probability low effect

Active Publication Date: 2021-02-19
辽宁省水稻研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional pedigree method selects the best breeding varieties from the separated offspring through the combination of two parents. The blast resistance phenotype depends on natural identification in the field or artificial inoculation identification, which is difficult to identify and has low accuracy.
Because they do not know the blast resistance genotype of the parents, the breeders are very blind in the selection of the parents, usually mass matching, massive selection of offspring, heavy workload and low breeding efficiency

Method used

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  • Dominant Molecular Marker of Rice Blast Resistance Gene pi67(t) and Its Application
  • Dominant Molecular Marker of Rice Blast Resistance Gene pi67(t) and Its Application
  • Dominant Molecular Marker of Rice Blast Resistance Gene pi67(t) and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Location of Rice Blast Resistance Gene Pi67(t)

[0031] Using the blast-resistant rice variety GY8 to cross the rice blast-susceptible variety Liaoxing 1, from F 2 In the first generation, the recombinant inbred line population RILs was constructed using the method of single seed transmission, and the population included 197 lines.

[0032] The above-mentioned obtained strains were planted in the rice blast induction nursery in Liaoning Province, and the resistance of 197 strains and two parents in the above-mentioned obtained population to rice blast was investigated for two consecutive years; the pathogenic response type and race were determined according to the national Evaluation was carried out according to the unified standard of the Joint Test Group of Physiological Races of Magnaporthe grisea (1980). The survey record standards are:

[0033]

[0034]

[0035] The genotypes of two parents and 197 lines were detected by 8K rice gene chips, and a ...

Embodiment 2

[0036] Example 2 Development of molecular markers co-segregated with rice blast resistance gene Pi67(t)

[0037] The rice line GY8 carrying the rice blast resistance gene Pi67(t) and the rice variety Liaoxing 1 not carrying the gene were resequenced by next-generation resequencing technology, and the sequencing data with an average coverage depth greater than 30× were obtained. By comparing the sequences of the two varieties, it was found that the two rice varieties had a 141bp insertion site in the localization region of the rice blast resistance gene Pi67(t), and the rice blast resistance gene Pi67(t) and the Indel site were on the chromosome location, such as figure 1 shown.

[0038] Depend on figure 1 It can be seen that the rice blast resistance gene Pi67(t) is located in the range of 10.18Mb-11.19Mb on rice chromosome 12, and the Indel site is located at 10.93Mb; compared with Liaoxing 1 that does not carry the gene Pi67(t), it carries There is a 141bp insertion in GY...

Embodiment 3

[0046] Example 3 Verification of molecular markers co-segregated with rice blast resistance gene Pi67(t)

[0047] F 2 In the population, the mixture of the rice blast fungus popular in Liaoning area was used to artificially inoculate the individual plants in the seedling stage at the seedling stage, and their resistance to rice blast was identified. and using the primer combination SEQ ID NO: 2 / SEQ ID NO: 3 for F 2 The genotype of each plant in the population was identified.

[0048] The specific test is as follows:

[0049] Using the donor variety GY8 of rice blast resistance gene Pi67(t) and the susceptible variety Liaoxing 1 as parents, F2 populations were constructed as test materials and planted in the greenhouse. The rice blast fungus ZA1, ZA9, ZB1 and ZF1 popular in Liaoning area were respectively transplanted on the oatmeal tomato juice agar medium, and cultured at 25-27°C for 7 days. After growing mycelia and cultivating moisture, the blast fungus will produce a l...

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Abstract

The invention, which belongs to the field of crop molecular genetic breeding science, particularly relates to a dominant molecular marker of a rice-blast-resistant gene Pi67 (t) and application thereof. The molecular marker is characterized in that 141bp insertion exists in a positioning region of a rice-blast-resistant gene Pi67 (t), and the nucleotide sequence is shown as SEQID NO. 1. Whether ato-be-detected rice sample carries the rice-blast-resistant gene Pi67 (t) or not can be accurately judged by detecting the molecular marker, so that the rice-blast-resistant rice variety breeding process is accelerated.

Description

technical field [0001] The invention belongs to the field of crop molecular genetics and breeding, and in particular relates to a dominant molecular marker of rice blast resistance gene Pi67(t) and an application thereof. Background technique [0002] Rice blast (Pyricularia grisea cav.) is a rice disease caused by Magnaporthe oryzae (Anamorphic: Pyricularia), and its distribution is very wide. According to statistics, it occurs in more than 80 countries in the world, and it can cause a 10%-20% reduction in production in general epidemic years, and it can reach 40%-50% in severe cases. Since the 1990s, the annual occurrence area of ​​rice blast in my country has been more than 3.8 million hm2, and the annual loss of rice has reached hundreds of millions of kilograms. With the simplification and centralization of varieties and the impact of climate, the harm of rice blast is becoming more and more serious. Although people have spent a lot of energy and material input on dis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 马作斌唐志强王昌华顾爽王丽丽赵明珠何娜付亮张丽颖郑文静
Owner 辽宁省水稻研究所
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