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A rice blast resistance gene pi67(t), its co-dominant molecular marker closely linked and its application

A rice blast resistance gene and molecular marker technology, applied in the field of crop molecular genetics and breeding, can solve the problems of single variety, large-scale planting, complex and changeable rice blast bacteria, and impact on food security, achieving low cost, good specificity, The effect of high accuracy

Active Publication Date: 2021-02-26
辽宁省水稻研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past 20 years, although great breakthroughs have been made in high-yield breeding, due to the complexity and changeability of the blast fungus, the narrow resistance spectrum of varieties, and the large-scale planting of single varieties, many excellent varieties have lost their resistance after several years of promotion. , seriously affecting my country's food security

Method used

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  • A rice blast resistance gene pi67(t), its co-dominant molecular marker closely linked and its application
  • A rice blast resistance gene pi67(t), its co-dominant molecular marker closely linked and its application
  • A rice blast resistance gene pi67(t), its co-dominant molecular marker closely linked and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Location of Rice Blast Resistance Gene Pi67(t)

[0031] Using the blast-resistant rice variety GY8 to cross the rice blast-susceptible variety Liaoxing 1, from F 2 In the first generation, the recombinant inbred line population RILs was constructed using the method of single seed transmission, and the population included 197 lines.

[0032] The above-mentioned obtained strains were planted in the rice blast induction nursery in Liaoning Province, and the resistance of 197 strains and two parents in the above-mentioned obtained population to rice blast was investigated for two consecutive years; the pathogenic response type and race were determined according to the national Evaluation was carried out according to the unified standard of the Joint Test Group of Physiological Races of Magnaporthe grisea (1980). The survey record standards are:

[0033] Level 0 disease-free……………………………………… High resistance (HR)

[0034] Grade 1 Brown dots the size of a needle tip...

Embodiment 2

[0044] Example 2 Development of Molecular Markers Tightly Linked to Rice Blast Resistance Gene Pi67(t)

[0045] The rice line GY8 carrying the rice blast resistance gene Pi67(t) and the rice variety Liaoxing 1 not carrying the gene were resequenced by next-generation resequencing technology, and the sequencing data with an average coverage depth greater than 30× were obtained. By comparing the sequences of the two varieties, it was found that there was a 60bp insertion / deletion site (Indel) in the localization region of the rice blast resistance gene Pi67(t) in the two rice varieties, such as figure 2 Shown, the position and sequence of rice blast resistance gene Pi67(t) and Indel locus on the chromosome, such as figure 1 , 2 shown.

[0046] Depend on figure 1 It can be seen that the rice blast resistance gene Pi67(t) is located within the 10.18Mb-11.19Mb range of rice chromosome 12, and the Indel site is located in the localization region; figure 2It can be seen that th...

Embodiment 3

[0054] Example 3 Verification of Molecular Markers Tightly Linked to Rice Blast Resistance Gene Pi67(t)

[0055] F 2 In the population, the mixture of the rice blast fungus popular in Liaoning area was used to artificially inoculate the individual plants in the seedling stage at the seedling stage, and their resistance to rice blast was identified. and using the primer combination SEQ ID NO: 2 / SEQ ID NO: 3 for F 2 The genotype of each plant in the population was identified.

[0056] The specific test is as follows:

[0057] Using the donor variety GY8 of rice blast resistance gene Pi67(t) and the susceptible variety Liaoxing 1 as parents, F2 populations were constructed as test materials and planted in the greenhouse. The rice blast fungus ZA1, ZA9, ZB1 and ZF1 popular in Liaoning area were respectively transplanted on the oatmeal tomato juice agar medium, and cultured at 25-27°C for 7 days. After growing mycelia and cultivating moisture, the blast fungus will produce a la...

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Abstract

The invention belongs to the field of crop molecular genetics and breeding, and specifically relates to a rice blast resistance gene Pi67(t), a co-dominant molecular marker closely linked thereto and its application. The molecular marker is a tightly linked molecular marker of the rice blast resistance gene Pi67(t), which is a nucleotide amplified from the total DNA of rice by using the primer combination SEQ ID NO: 2 and SEQ ID NO: 3 By detecting the molecular marker, it can be accurately judged whether the rice sample to be tested carries the rice blast resistance gene Pi67(t), and the breeding process of rice blast resistance rice varieties can be accelerated.

Description

technical field [0001] The invention belongs to the field of crop molecular genetics and breeding, and specifically relates to a rice blast resistance gene Pi67(t), a co-dominant molecular marker closely linked thereto and its application. Background technique [0002] Rice is one of the most important crops in our country, and it is of vital significance to ensure our country's food security. Rice blast caused by Magnaporthe oryzae is one of the diseases that seriously damage rice production. Once the disease occurs, it not only reduces rice yield, but also affects rice quality and brings serious losses to rice production. The annual cultivation area of ​​japonica rice in northern my country is more than 90 million mu, accounting for about 60% of the national japonica rice production area. In the past 20 years, although great breakthroughs have been made in high-yield breeding, due to the complexity and changeability of the blast fungus, the narrow resistance spectrum of v...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 郑文静王昌华马作斌王丽丽唐志强顾爽赵明珠何娜付亮张丽颖张继东
Owner 辽宁省水稻研究所
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