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Method for measuring pig gene polymorphism and relevance between pig gene polymorphism and sow reproduction traits

A technology of gene polymorphism and determination method, applied in the field of determination of pig gene polymorphism and its correlation with reproductive traits of sows, can solve problems such as blanks

Active Publication Date: 2016-03-02
安徽大自然种猪股份有限公司
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Problems solved by technology

Relevant experiments have proved that RBP4 and BMP7 genes have a certain correlation with the reproductive performance of Landrace and Large White pigs, but the research on RBP4 and BMP7 genes in Anhui local pig breeds is almost blank

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  • Method for measuring pig gene polymorphism and relevance between pig gene polymorphism and sow reproduction traits
  • Method for measuring pig gene polymorphism and relevance between pig gene polymorphism and sow reproduction traits
  • Method for measuring pig gene polymorphism and relevance between pig gene polymorphism and sow reproduction traits

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Embodiment 1

[0077] A method for determining the correlation between pig gene polymorphisms and reproductive traits of sows, comprising the steps of:

[0078] (1) collecting ear tissue samples of adult fertile sows;

[0079] (2) DNA extraction from ear sample tissue;

[0080] (3) PCR amplification of retinol-binding protein, that is, RBP4, and bone morphogenetic protein, that is, the BMP7 gene target fragment;

[0081] (4) Single-strand conformational polymorphism of the target fragment, that is, SSCP analysis;

[0082] (5) Two-way sequencing of PCR amplification products;

[0083] (6) Analysis of population genetic characteristics;

[0084] (7) Determination of reproductive performance;

[0085] (8) The relationship between the detected genetic variation of RBP4 and BMP7 genes and the reproductive traits of sows was analyzed by mathematical model.

[0086] According to the correlation analysis between the single nucleotide polymorphism of the target gene (singlenucleotide polymorphis...

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Abstract

The invention discloses a method for measuring pig gene polymorphism and relevance between pig gene polymorphism and sow reproduction traits. The method includes the following steps of firstly, collecting a pig ear tissue sample; secondly, extracting ear tissue DNA; thirdly, amplifying RBP4 and BMP7 gene target segments through a PCR; fourthly, conducting SSCP analysis; fifthly, conducting bidirectional sequencing on a PCR amplification product; sixthly, conducting population genetics characteristic analysis; seventhly, measuring reproductive performance; eighthly, analyzing the relevance between detected RBP4 and BMP7 gene heritable variation and sow reproduction trait indexes through a mathematical model. The method has the advantages of being high in detecting efficiency and reliable in result.

Description

technical field [0001] The invention relates to a method for determining the correlation between gene polymorphism and reproductive traits, in particular to a method for determining the correlation between gene polymorphism and reproductive traits of sows. Background technique [0002] In the current pig industry, pig breeding in our country often falls into a vicious circle, that is, "introduction-degeneration-reintroduction-re-degeneration". Academician Wu Changxin believes that there are two reasons for this strange circle phenomenon. On the one hand, When the breeding pigs are introduced, they have fallen behind, and other countries will not give us the best pigs; second, the improvement effect of breeding selection after the introduction of breeding pigs is not as good as that of foreign countries. In order to get out of this vicious circle, the more feasible solution recognized in the industry is: while introducing breeds, effective breeding methods should be used to i...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 殷宗俊丁月云张晓东朱鹏张威张梦琦付坤王源朗朱树娇张德磊
Owner 安徽大自然种猪股份有限公司
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